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Updated: Jun 27, 2025

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

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对肝素结合蛋白的DNA阿普塔默的查和应用

Xi Zhou^1,2, Yingying Cao^1,2, Xiaocui Huang³

¹Fujian Key Laboratory of Aptamers Technology, Fuzhou General Teaching Hospital (the 900th Hospital), Fujian University of Traditional Chinese Medicine, Fuzhou 350025, China.

Molecules (Basel, Switzerland)

|April 27, 2024

概括

这项研究开发了一种基于aptamer的新型生物传感器,用于快速检测肝素结合蛋白 (HBP),这对于败血症诊断至关重要. 这种新方法为传统技术提供了一种敏感和特定的替代方案,改善了临床干预的及时性.

科学领域:

生物化学生物化学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

快速检测肝素结合蛋白 (HBP) 对于有效的败血症干预至关重要.
现有的基于抗体的方法是复杂的,缓慢的,不能满足紧急的临床需求.
DNA 体为敏感和特定的HBP检测提供了一个有前途的替代方案.

研究的目的:

为了选和识别HBP特异性DNA吸收体.
开发一种基于aptamer的光生物传感器,用于定量检测HBP.
评估生物传感器的性能,以便在败血症诊断中潜在的临床应用.

主要方法:

使用基于亲和力的方法选HBP特异性DNA吸收体.
设计一种以亚酶为媒介的DNA放大策略,以ExoIII为辅助,以循环模板为基础.
使用SYBR GreenII进行信号检测的光生物传感器的开发.

主要成果:

鉴定出具有高亲和力的HBP亚体 (Apt-01,Apt-02,Apt-13) ,其K_D值低至1.04nmol/L.
开发了一种使用Apt-01的光生物传感器,其线性检测范围为0.01-10nmol/L.
为HBP达到0.0056nmol/L的低检测极限,表明高灵敏度.

结论:

开发的以aptamer为基础的光生物传感器为HBP检测提供了灵敏,特异和快速的方法.
这种方法克服了用于临床诊断的传统抗体基试验的局限性.
生物传感器具有显著的潜力,可以改善早期败血症诊断和患者管理.

关键词:

它们是Aptamers.生物传感器生物传感器氨酸结合蛋白质 - 氨酸结合蛋白质滚动圈放大放大滚动圈放大放大这是一种血症.

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