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Updated: Jun 27, 2025

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Published on: May 2, 2011

在用重组RBD-IgG化学蛋白免疫后,增强免疫性和对SARS-CoV-2的保护作用

Mariângela de Oliveira Silva¹, Maria Fernanda Castro-Amarante², Alexia Adrianne Venceslau-Carvalho²

¹Laboratory of Antigen Targeting to Dendritic Cells, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.

|April 27, 2024

概括

这项研究将SARS-CoV-2受体结合域 (RBD) 与IgG抗体融合在一起,创建了一种新型亚单元疫苗. 这种增强的疫苗抗原改善了免疫反应,并为小鼠的致命SARS-CoV-2挑战提供了充分的保护.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学
病毒学病毒学

背景情况:

由于SARS-CoV-2的全球影响,需要有效的疫苗.
SARS-CoV-2受体结合域 (RBD) 是中和抗体的关键目标.
由于RBD的免疫性较低,因此需要采取提高其免疫性质的策略.

研究的目的:

为了评估由基因融合RBD与小鼠IgG抗体创建的子单位疫苗抗原.
评估新型RBD-IgG抗原的免疫性和保护效果.

主要方法:

在小鼠中皮下注射RBD-IgG抗原.
测量RBD特异性IgG标位和病毒中和抗体标位 (使用伪病毒和活的SARS-CoV-2).
在K18-hACE2小鼠中对致命的SARS-CoV-2挑战进行保护的评估.

主要成果:

RBD-IgG的使用导致血液中的蛋白质存在时间延长,并增强了RBD特定的IgG标位.
用RBD-IgG免疫接种增加了病毒中和抗体标位.
用RBD-IgG免疫的K18-hACE2小鼠完全受到致命的SARS-CoV-2挑战的保护,显示控制的体重减轻和减少病毒载荷.

结论:

RBD与IgG的基因融合增强了抗原免疫性,并促进了病毒中和抗体的产生.
IgG 嵌合抗原是改善 SARS-CoV-2 亚单元疫苗性能的一种有希望的方法.
这一战略为开发更有效的针对SARS-CoV-2的疫苗提供了潜力.

关键词:

在 IgG 融合和 IgG 融合过程中.这就是SARS-CoV-2病毒.再组合的RBD可以使用.部分单位的疫苗.

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