从人类,动物和环境标本检测四种冠状病毒的比较性能
Supaporn Wacharapluesadee1,2, Nattakarn Thippamom1, Piyapha Hirunpatrawong1,2
1Thai Red Cross Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand.
Viruses
|April 27, 2024
概括
使用联合Q-和X-CoV PCR分析的新分子方法提高了在人类,动物和环境样本中早期检测新型冠状病毒 (CoV),提高了诊断灵敏度.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子诊断学 分子诊断
- 环境微生物学 环境微生物学
背景情况:
- 新出现的冠状病毒 (CoV) 对人类和动物健康构成重大威胁,需要强有力的检测方法.
- 野生动物水库是CovV溢出事件的主要来源,导致广泛的呼吸道疾病.
- 早期识别已知和新型冠状病毒对于疾病控制和预防至关重要.
研究的目的:
- 评估和比较三种已发表的泛冠状病毒 (CoV) PCR测定 (Q-,W-和X-CoV PCR) 的性能.
- 评估这些试验在各种样本类型中检测CoV的诊断效用,包括人类,废水,蝙蝠和鸟类标本.
- 为了确定测试的最佳组合,以进行敏感和可靠的CovV检测.
主要方法:
- 已存档的标本 (50人,46废水,28蝙蝠,17鸟类) 使用三个不同的泛冠状病毒PCR试验进行了测试:Q-CoV PCR,W-CoV PCR和X-CoV PCR.
- 测试性能与四种CovV属进行了评估,以评估检测范围.
- 对不同样本类型和CoV类型的敏感性和特异性进行了比较.
主要成果:
- 在X-CoV的PCR测定证明了检测所有四个CoV属的能力,而Q-和W-CoV的PCR测定未能检测delta-CoV (δ-CoV) 属.
- 在人类标本中检测率为42.0% (Q-CoV),18.0% (W-CoV) 和42.0% (X-CoV).
- 在废水样本中检测率为65.22% (Q-CoV),13.04% (W-CoV) 和58.70% (X-CoV).
- 对于Q-CoV PCR,X-CoV PCR的敏感性与蝙蝠CoV检测的Q-CoV PCR相似.
结论:
- 对X-CoV的PCR测定在检测广泛的Cov类病毒中是有效的.
- 结合Q-和X-CoV PCR测试,显著提高了检测新型冠状病毒的敏感性.
- 这种综合方法有助于减轻假阴性结果,这对于早期识别和监测新出现的冠状病毒至关重要.
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