在两个贝塔冠状病毒的实验进化过程中,缺陷基因组的积累动力学
Julia Hillung1, María J Olmo-Uceda1, Juan C Muñoz-Sánchez1
1Instituto de Biología Integrativa de Sistemas (I2SysBio), CSIC-UV, Catedrático Agustín Escardino Benlloch 9, Paterna, 46980 Valencia, Spain.
Viruses
|April 27, 2024
概括
缺陷的病毒基因组 (DVGs) 在betacoronavirus进化过程中变得更加多样化和丰富. 感染的多重性 (MOI) 影响DVG的形成和特征,影响病毒的复制和进化.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 缺陷病毒基因组 (DVGs) 是由病毒编码复制酶产生的异常RNA基因组.
- DVG需要辅助病毒进行复制,但可以影响病毒动态,免疫反应和进化.
- 与标准病毒基因组一起DVG的进化动态尚未完全理解.
研究的目的:
- 调查DVG在贝塔冠状病毒长期实验进化过程中的积累动态和演变.
- 确定多重感染 (MOI) 对DVG形成和特征的影响.
主要方法:
- 在高和低MOI的细胞培养中,人类冠状病毒OC43 (HCoV-OC43) 和小鼠肝炎病毒 (MHV) 的实验进化.
- 在定期的时间间隔下进行RNA测序 (RNA-seq).
- 重建和分析DVG序列及其积累动态.
主要成果:
- 随着DVG的演变,多样性和丰富性增加,删除和插入是常见的.
- MOI显著影响了DVG的丰富性和大小分布,特别是在HCoV-OC43.3中.
- 在高MOI下编码结构性和辅助蛋白的区域中确定了特定的删除热点.
- 与HCoV-OC43.3相比,MHV在DVG大小分布中显示出较少明显的MOI依赖变异.
结论:
- 贝塔冠状病毒的演变导致广泛的DVG形成.
- MOI 是调节DVG演变和特征的关键因素.
- 细胞和病毒特异性因素也会在感染期间对DVG动态产生影响.
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