患有慢性肠衰竭的成年人肠道微生物群
Loris Pironi1, Federica D'Amico2, Mariacristina Guidetti3
1Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; Centre for Chronic Intestinal Failure, IRCCS AOUBO, Bologna, Italy.
Clinical nutrition (Edinburgh, Scotland)
|April 27, 2024
概括
患有慢性肠衰竭 (CIF) 的成年人表现出显著的肠道失调. 微生物概况因病因而异,包括短肠综合征 (SBS),并与疾病的严重程度有关.
科学领域:
- 胃肠病学 胃肠病学
- 微生物组研究 微生物组研究
- 临床营养学 临床营养学
背景情况:
- 慢性肠衰竭 (CIF) 影响了许多成年患者,具有不同的潜在病理生理机制.
- 短肠综合征 (SBS) 是CIF的一个主要原因,按解肠类型分类 (凝肠结肠 - SBS-2,凝肠结肠 - SBS-1).
- 有限的数据存在于SBS-1和CIF中的便微生物群,由于肠道动力不良 (DYS) 或粘膜疾病 (MD).
研究的目的:
- 调查和分析慢性肠衰竭 (CIF) 的各种病理生理机制的成年患者的便微生物群.
- 为了比较不同的CIF病因和健康对照 (HC) 之间的肠道微生物组成和多样性.
- 识别与SBS亚型,DYS和MD相关的特定微生物特征.
主要方法:
- 一项涉及61名患有CIF (SBS-1,SBS-2,DYS,MD) 的成年患者和年龄/性别匹配的健康对照 (HC) 的横截面研究.
- 便样本采集并使用16S rRNA amplicon测序进行分析.
- 评估了微生物群的多样性 (alpha和beta) 和分类构成,并在各组之间进行了比较.
主要成果:
- 与HC相比,SBS-1,SBS-2和MD患者的α多样性明显较低;DYS患者没有差异.
- 贝塔多样性分析显示,SBS-1,SBS-2和DYS组的分别聚类,将它们从HC和彼此分开.
- 与HC相比,CIF群体表现出变化的系级组成,乳酸菌属和肠杆菌属增加,而Lachnospiraceae和Ruminococcaceae减少.
- 在SBS组中的微生物群概况与腹腔营养需求和CIF持续时间相关联.
结论:
- 患有CIF的成年患者有明显的肠道失生症.
- 特定的微生物特征与CIF的独特病理生理机制有关.
- 短肠综合征的严重程度和持续时间会影响观察到的肠道微生物群组成.
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