齐林宁配方通过通过AHR/NF-κBp65轴抑制炎症和肠道屏障功能障碍来缓解DSS诱导的大肠炎
Rui Zhou1, Kaiwen Huang1, Simin Chen1
1School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, PR China.
概括
齐林宁配方 (ZLN) 通过调节AHR/NF-κBp65轴,减少炎症和修复肠道屏障,有效治疗性结肠炎 (UC). 这项研究为ZLN提供了机制基础.
科学领域:
- 胃肠病学和药理学 胃肠病学和药理学
- 免疫学 免疫学 免疫学
- 代谢学 代谢学 代谢学
背景情况:
- 性结肠炎 (UC) 治疗需要修复肠道屏障并减少持续性炎症.
- 林配方 (ZLN) 是一种传统的中医药,用于肠炎和痢疾,但其在UC中的机制尚不清楚.
研究的目的:
- 研究ZLN对UC的治疗作用.
- 通过代谢学和实验验证,阐明UC中ZLN的潜在机制.
主要方法:
- 用于评估ZLN的疗效,使用了一种硫酸 (DSS) 诱导的大肠炎的小鼠模型.
- 代谢分析 (UPLC-Q-TOF-MS/MS) 确定了大肠炎中代谢途径的改变.
- 与AHR/NF-κBp65轴相关的蛋白质和mRNA表达被使用qPCR和西式斑点分析.
- 在体外实验中探索了ZLN的活性成分与AHR和NF-κBp65.5的相互作用.
主要成果:
- 治疗ZLN改善了临床和组织学评分,减少了炎症标志物 (MPO,IL-1β,TNF-α,IL-18) 并保护了DSS诱导的大肠炎的肠壁.
- 代谢学揭示了ZLN对酸和酸代谢的调节,这表明它与AHR/NF-κBp65轴有联系.
- ZLN抑制了NF-κBp65信号传递,减少了促炎性细胞因子,增加了AHR核转位,并促进了IL-22的表达.
- 实验室研究证实,ZLN激活AHR并抑制NF-κBp65,从而产生抗结肠炎作用.
结论:
- 齐林宁配方 (ZLN) 通过调节AHR/NF-κBp65信号通路来缓解性结肠炎.
- 这项研究为ZLN在UC的临床应用提供了机制的理解和理论基础.
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