对酵母Tbf1作为非典型的端粒重复结合因子的结构和功能见解
Zhenfang Wu1, Xin Gu2, Lin Zha1
1Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.
Structure (London, England : 1993)
|April 27, 2024
概括
裂变酵母中的端粒重复结合因子1 (Tbf1) 通过其TRFH和Myb-L域结合端粒DNA. 与其他TRF不同的是,Tbf1缺少庇护式对接图案,解释了其独特的功能.
科学领域:
- 分子生物学分子生物学
- 结构生物学是结构生物学.
- 遗传学 是一个遗传学.
背景情况:
- 端粒重复结合因子1 (Tbf1) 与端粒重复结合因子 (TRFs) 具有结构上的相似性,并影响端粒恒温和核糖体调节.
- 与其他TRF相比,Tbf1的独特功能背后的分子机制尚未完全理解.
研究的目的:
- 为了阐明Schizosaccharomyces pombe Tbf1 (spTbf1) 功能的结构基础.
- 研究spTbf1域在端粒DNA结合和蛋白质相互作用中的作用.
主要方法:
- 进行X射线晶体学以确定spTbf1的TRF同质 (TRFH) 和Myb-L域的结构.
- 生物化学分析分析spTbf1的DNA结合能力和相互作用.
主要成果:
- 确定了spTbf1 TRFH和Myb-L域的晶体结构.
- 通过TRFH介导的同质化对于spTbf1稳定性至关重要.
- 在 spTbf1 的 TRFH 域中,缺少保护性对接动机,用于 shelterin 复合相互作用.
- spTbf1通过其TRFH,Myb-L域和循环区域的协调努力来识别S. pombe端粒双链DNA.
结论:
- 在真菌中,spTbf1作为非典型的端粒重复结合因子起作用.
- 揭示了spTbf1独特的作用的结构基础,它与庇护所组成部分不同.
- 这些发现为含有TRFH的端粒蛋白的进化提供了洞察力.
相关概念视频
Telomeres and Telomerase
23.3K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
23.3K
Replication in Eukaryotes
13.7K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.7K
Protein Complexes with Interchangeable Parts
2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K
Chromosome Structure
22.7K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
22.7K
Replicative Cell Senescence
3.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.6K
General Transcription Factors
5.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K


