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在常见的板读器和显微镜上进行动态模式通道测试的协议

Emery Smith¹, Louise Dickson², Philip Pickford²

¹The Herbert Wertheim UF Scripps Institute for Biomedical Innovation and Technology, Department of Molecular Medicine, 130 Scripps Way #1A1, Jupiter, FL 33458, United States.

SLAS discovery : advancing life sciences R & D

|April 27, 2024

概括

这项研究将通道测试适用于常见的终点板读取器,使通道调节器选更容易获得. 的快照测定使得更广泛的实验室使用和GIRK通道激活剂的识别.

科学领域:

生物化学生化学
药理学药理学是指药理学的学科.
细胞生物学细胞生物学

背景情况:

基于光的通道测试通常需要专门的,昂贵的动力板阅读器.
先进设备的有限可用性阻碍了广泛的通道研究和药物发现.

研究的目的:

为了适应动力通道测定用于常见的终点板读取器.
为了使通道调节器的识别得到更广泛的访问.
为了验证用于选GIRK通道激活器的适应试验.

主要方法:

将动态分子器件FLIPR协议调整为终端格式.
在终点盘阅读器 (例如,BMG Labtech PHERAstar) 上使用了明亮快照试验.
选了Sigma LOPAC 1,280化合物库,以识别CHO细胞中的GIRK通道激活器.

主要成果:

证明了功能能力和极好的Z'值在终点板读取器上.
从复合选集合中成功识别了相关结果.
在高内容读取器上验证了试验,显示了空间分辨率单细胞分析的潜力.

结论:

与终点兼容的通道测定是灵活的,并且可以在常见的板读器上访问.
这种调整后的测定方法有助于更广泛地识别通道调节器.
该方法增强了标准实验室设备在药物发现中的实用性.

关键词:

细胞洞察细胞洞察在FLIPR上看到的吉尔克·吉尔克 (GIRK) 是一个在HTS中,HTS就是HTS.菲拉斯塔尔是菲拉斯塔尔的一个星球.快照中的快照.

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