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细胞类型和性别特定的节奏基因表达在核中

Lauren M DePoy^1,2, Kaitlyn A Petersen^1,2, Wei Zong³

¹Department of Psychiatry, Translational Neuroscience Program, University of Pittsburgh School of Medicine, 15219, Pittsburgh, PA, USA.

Molecular psychiatry

|April 27, 2024

概括

鼠标核突变 (NAc) 细胞类型中的日间节律揭示了核心时钟基因在性别和细胞类型之间达到类似的峰值. 时钟控制的基因显示性别特定的时间,影响奖励通路,并可能延迟女性的节奏.

科学领域:

神经科学是一个神经科学.
时间生物学时间生物学
基因组学就是基因组学.

背景情况:

循环节律对健康至关重要,与精神疾病等疾病相关的干扰.
之前的研究表明,昼夜基因与核聚集体 (NAc) 神经元行为有关,特别是多巴胺D1表达中等棘手神经元 (MSN).
在NAc MSN中,日间转录表达节奏仍然在很大程度上没有特征.

研究的目的:

识别和描述D1-和D2-表达NAc神经元中的昼间节律转录.
为了将这些节奏与来自雄性和雌性小鼠的NAc同质和天体细胞样本中的节奏进行比较.
为了研究转录节律性和相关的生物学途径的性别差异.

主要方法:

单细胞RNA测序用于分析D1-MSN,D2-MSN和来自雄性和雌性小鼠NAc的天体细胞中的转录表达.
使用生物信息学算法识别了节奏转录.
进行了途径丰富分析,以了解节律基因的功能.

主要成果:

所有分析的细胞类型 (D1-MSNs,D2-MSNs,星球细胞) 都表现出白天转录节奏.
核心时钟基因在细胞类型和性别之间显示类似的峰值表达时间.
时钟控制的成绩单显示性别特定的峰值表达,女性显示延迟,可能影响奖励通路.
途径丰富分析揭示了节律基因功能的细胞类型和性别特异性差异.
发现MSN分类的关键标记是节奏性的,这给细胞类型特定研究带来了挑战.

结论:

纳克表现出不同的细胞类型和性别的复杂的日间转录节奏.
时钟控制的基因节律中的性别差异可能是奖励处理中的性别特异性的基础.
MSN标记物的节奏性需要在未来的NAc研究中仔细考虑采样时间.
这项研究提供了NAc细胞节律的全面地图,增强了我们对它们在行为和疾病中的作用的理解.

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