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通过使用工程化Cupriavidus necator H16的石化自营发酵,将二氧化碳提升为白醇

Yongjae Jang¹, Yeon Ji Lee¹, Gyeongtaek Gong^1,2

¹Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea.

Microbial cell factories

|April 27, 2024

概括

这项研究设计了Cupriavidus necator H16,从二氧化碳中产生复星,证明了可持续的生物制造方法. 工程细菌成功地将二氧化碳转化为有价值的多化合物.

科学领域:

微生物生物技术微生物生物技术
合成生物学合成生物学
代谢工程是代谢工程.

背景情况:

在工业生物制造中利用二氧化碳 (CO2) 为传统碳水化合物原料提供了一个可持续的替代方案.
库普里亚维杜斯 (Cupriavidus necator H16) 是一种光自营菌,由于其遗传处理性和碳储存能力,它显示出作为基于C1的生物制造平台的前景.

研究的目的:

通过人工的烯胺路径,对Cupriavidus necator H16进行自性产生复星的工程设计.
建立可持续的生物制造工艺,从二氧化碳中获得有价值的多化合物.

主要方法:

进行复醇生物合成的异质基因的实施:铁氨酸酶 (TAL),4-甲基CoA酶 (4CL) 和斯蒂尔本合成酶 (STS).
遗传修饰包括过度表达的乙-CoA碳糖酶 (ACC),破坏的聚基酸盐 (PHB) 合成途径,并增加STS基因的副本数量.
使用二氧化碳和氨酸作为基质的石化自营发酵.

主要成果:

经过工程设计的C. necator H16通过人工烯胺路径成功产生了复星.
过度表达ACC,扰乱PHB合成,增加STS基因拷贝增强了复星的产生.
最终的CR-5菌株从二氧化碳和氨酸中产生了1.9毫克/升的复星.

结论:

这项研究代表了首份关于利用工程C. necator H16菌株将二氧化碳价值化为多化合物的报告.
证明了C. necator H16作为可持续化学生产的多功能平台的潜力.
突出了使用石化自营发酵来从二氧化碳中生产高价值化合物的可行性.

关键词:

红虫 (Cupriviadus necator) H16 H16 是一种有毒的植物.二氧化碳的价值化在Lithoautotrophic生产中.复星醇是一种

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