脊髓灰质炎和COVID-19:一个重复的历史
1Enteric Viral Infections, Laboratory 'Cantacuzino' Medico Military National Institute of Research and Development, 050096 Bucharest, Romania; Department of Microbiology III - Emergency University Hospital Bucharest, Faculty of Medicine, 'Carol Davila' University of Medicine and Pharmacy, 050098 Bucharest, Romania
Journal of water and health
|April 28, 2024
概括
污水监测检测到27%的样本中有SARS-CoV-2,79%的样本中有肠道病毒. 这种废水监测证实了罗马尼亚的废水监测.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 公共卫生监督是对公共卫生的监督.
背景情况:
- 废水监测对于早期检测生态系统中的肠道病毒和SARS-CoV-2等病毒病原体至关重要.
- 监测污水中的病毒循环,可以了解社区层面的传播动态.
研究的目的:
- 评估罗马尼亚污水中的肠道病毒和SARS-CoV-2的共流.
- 评估多重实时PCR在废水中同时检测病毒的实用性.
- 通过肠道病毒监测,确认罗马尼亚的无脊髓灰质炎状态.
主要方法:
- 从2021年9月到2023年4月,收集和分析了81个污水样本.
- 使用多重实时PCR试验 (生物火膜阵列呼吸系统2.1 Plus面板) 进行分子检测.
- 按照世卫组织的指导方针在细胞培养线上隔离肠道病毒.
主要成果:
- 在22% (27%) 的样本中检测到严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2).
- 在 64% (79%) 的样本中通过分子方法检测到人类犀利病毒/肠道病毒.
- 33.33% (27) 的样本对非脊髓灰质炎肠道病毒呈阳性;没有分离的脊髓灰质炎病毒菌株.
结论:
- 废水监测有效监测SARS-CoV-2和肠道病毒的联合循环.
- 多重PCR提供了一种快速的方法来检测污水中的病原体,这对于应急反应至关重要.
- 这项研究证实了罗马尼亚的持续无脊髓灰质炎状态.
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