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从Aegilops物种内进的耐基因Lr53和Yr35的映射和表征

Binyang Xu^1,2, Tao Shen^1,3, Hong Chen²

¹National Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in Weifang, Weifang, 261325, Shandong, China.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

|April 28, 2024

概括

新的抗基因Lr53和Yr35成功地被映射到面包小麦染色体6BS上. 这些基因来自野生亲属的入,提供对破坏性小麦生疾病的持久抵抗力,有助于繁殖努力.

科学领域:

植物遗传学和育种.
保护作物的作物保护.
分子生物学分子生物学

背景情况:

小麦叶和条纹生是对小麦生产的重大全球威胁.
开发耐药小麦品种是疾病管理的关键可持续战略.

研究的目的:

在面包小麦中识别和绘制耐基因Lr53和Yr35的染色体位置.
调查这些内进抗性基因的起源和有效性.

主要方法:

使用SNP分析,序列比较和细胞遗传学测试来确定耐药基因的起源.
为了精确的基因映射,使用了大量的重组群体 (3892 个配体) 和ph1b突变.
包括pku6B3127在内的分子标记物被开发用于基因检测和监测链接拖延.

主要成果:

耐基因Lr53和Yr35被映射到染色体臂6BS的6.03Mb端粒区域.
Lr53表现出对中国叶种的广泛抵抗力,而Yr35则对特定的条纹种有效.
一项共同分离分析证实了Lr53和Yr35在映射区域内的密切联系.
一个发达的入侵线和减少的外星人部分保持了高的阻力水平,最大限度地降低了连接阻力.

结论:

该研究成功地将Lr53和Yr35映射到来自Aegilops物种的小麦染色体6BS上.
一个紧密联系的标记物,pku6B3127,可以促进这些抗性基因在育种计划中的部署.
开发的入侵线和标记物将加速将耐久耐性引入商业小麦品种.

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