使用拉曼光谱和间接硬模拟 (IHM) 进行生物工艺在线监测和控制
David Heinrich Müller1, Marieke Börger1, Julia Thien1
1Institute of Technical Thermodynamics, RWTH Aachen University, Aachen, Germany.
Biotechnology and bioengineering
|April 28, 2024
概括
拉曼光谱与间接硬模拟 (IHM) 结合,提供高效的生物过程监测和控制. 这种强大的过程分析技术 (PAT) 工具简化了在酵母发酵中对葡萄糖和乙醇测量的校准.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 频谱学是一种光谱学.
背景情况:
- 过程分析技术 (PAT) 对于优化生物工艺生产率至关重要.
- 拉曼光谱是一种常见的PAT工具,但其数据评估和模型校准可能是复杂的.
- 间接硬模型 (IHM) 为简化拉曼光谱分析提供了一个解决方案.
研究的目的:
- 为了证明拉曼光谱与IHM结合用于生物过程控制的能力.
- 评估这种综合方法在Saccharomyces cerevisiae发酵中控制葡萄糖质量分数的性能.
- 将校准工作和控制质量与传统的统计模型进行比较.
主要方法:
- 开发和应用一个间接硬模型 (IHM) 预测模型.
- 拉曼光谱与IHM的整合,用于对葡萄糖和乙醇进行非侵入性在线监测.
- 使用IHM-拉曼数据作为简单的开关血糖控制器的输入.
- 进行不同的葡萄糖设定点的酵母发酵.
主要成果:
- 拉曼光谱和IHM组合成功实现了对葡萄糖和乙醇的非侵入性直线监测.
- 该方法证明了在Saccharomyces cerevisiae发酵中有效控制葡萄糖质量分数.
- 过程控制质量与统计模型相比,校准工作量显著减少.
- 成功管理了两种具有不同葡萄糖设定点的发酵.
结论:
- 拉曼光谱和IHM的结合是用于生物过程监测和控制的强大PAT工具.
- 与统计模型相比,这种方法显著减少了校准工作量.
- 它为优化生物工艺生产率提供了一种实用而高效的方法.
相关概念视频
01:26Raman Spectroscopy Instrumentation: Overview
332
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
332
01:20Raman Spectroscopy: Overview
372
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
372