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磁性修饰的菌体触发的ATP释放被激活EXPAR-CRISPR/Cas14a系统用于 Burkholderia pseudomallei 的视觉检测

Juan Yao¹, Zhang Zhang², Hua Pei³

¹NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical University, Haikou, Hainan, 571199, PR China; Nanobiosensing and Microfluidic Point-of-Care Testing, Key Laboratory of Luzhou, Department of Clinical Laboratory, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, 646000, PR China.

Biosensors & bioelectronics

|April 28, 2024

概括

一个新的生物传感器使用菌体溶解和ATP释放检测Burkholderia pseudomallei. 这种方法提供了快速,灵敏的检测,这对于控制乳腺炎和环境监测至关重要.

科学领域:

微生物学微生物学
生物传感器技术技术
分子诊断学分子诊断

背景情况:

Burkholderia pseudomallei会导致蜂发病,这是一种致命的动物性传染病,在全球范围内日益蔓延.
对B. pseudomallei的快速和敏感的检测方法对于环境监测和感染控制至关重要.

研究的目的:

开发一种创新的生物传感器,用于定量检测可行的Burkholderia pseudomallei.
为了利用菌体诱导的溶解和ATP释放来产生信号.

主要方法:

性细菌体 (vB_BpP_HN01) 和磁性纳米粒子被用于特定捕获和丰富B. pseudomallei.
在溶解后释放的腺三酸盐 (ATP) 被使用EXPAR-CRISPR级联反应检测到用于信号放大.
光信号输出与细菌度相关.

主要成果:

生物传感器展示了一个动态检测范围从10^2到10^7 CFU mL−1.1.
在80分钟内达到45 CFU mL-1的检测极限 (LOD).
该系统在B. pseudomallei检测方面表现出卓越的性能.

结论:

开发的菌体亲和生物传感器为快速诊断B. pseudomallei.提供了一个强大的平台.
该方法适用于现场环境监测和感染控制应用.
信号输出兼容性提高了其在不同监控平台上的多功能性.

关键词:

双倍放大功率的扩大.指数级放大反应的反应是指数级的.全球流行率全球流行率梅里奥多症是什么意思在现场进行环境监测.

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相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...