在腹膜区域的Connexin-36阳性间隙连接处,GABA神经元维持阿片类药物依赖
Geith Maal-Bared1, Mandy Yee1, Erika K Harding2
1Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada.
The European journal of neuroscience
|April 28, 2024
概括
在腹膜区域 (VTA) 的GABA神经元中的Connexin-36 (Cx36) 对于成的动机转换至关重要. 阻止Cx36会使药物依赖的动物恢复到无毒状态,影响快乐和寻求缓解的行为.
科学领域:
- 神经科学是一个神经科学.
- 成研究 研究成研究
- 分子生物学分子生物学
背景情况:
- 药物依赖将动机从寻求快乐转移到寻求缓解.
- 腹部体区域 (VTA) 的GABA神经元连接这些动机的独特途径.
- 寻找阿片类药物涉及到不同的神经通路在天真与依赖的个体.
研究的目的:
- 研究VTA GABA神经元中连xin-36 (Cx36) 在药物依赖的动机转换中的作用.
- 确定Cx36是否调解了药物寻找的基础神经通路中的过渡.
主要方法:
- 在阿片类药物依赖和戒断 (ODW) 的老鼠的VTA中注射了Cx36阻断剂 (美floquine).
- 使用了缺少Cx36在VTA GABA神经元中的条件淘汰赛小鼠.
- 在VTA GABA神经元中进行病毒介导的Cx36救援.
主要成果:
- 在ODW大鼠中阻断Cx36使它们恢复到类似于药物先验的状态,使TPP成为阿片类CPP的必要条件,并消除了戒断厌恶.
- 在VTA GABA神经元中缺少Cx36的小鼠仍然处于一种永久的无毒状态,具有DA-独立的阿片类CPP并且没有戒断厌恶.
- 在VTA GABA神经元中恢复Cx36恢复了对ODW状态的敏感性,与DA依赖的阿片类CPP.
结论:
- 在VTA GABA神经元中的Cx36对于在阿片类药物依赖期间激励回路的切换至关重要.
- 由Cx36调解的VTA间隙连接对于成的神经生物学基础至关重要.
- 这些发现为成电路模型提供了新的见解.
相关概念视频
Gap Junctions
52.9K
Multicellular organisms employ a variety of ways for cells to communicate with each other. Gap junctions are specialized proteins that form pores between neighboring cells in animals, connecting the cytoplasm between the two, and allowing for the exchange of molecules and ions. They are found in a wide range of invertebrate and vertebrate species, mediate numerous functions including cell differentiation and development, and are associated with numerous human diseases, including cardiac and...
52.9K
Contact-dependent Signaling
44.6K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Gap Junctions
In animal cells, gap junctions are formed...
44.6K
Drug Abuse and Addiction: Pharmacological Phenomena
467
Drug dependence, abuse, and addiction are complex phenomena that can precipitate various abnormal states. Physical dependence refers to a state of pharmacological adaptation to a drug. This adaptation often results in tolerance—a reduced response to the drug after repeated administrations. When the drug use is abruptly stopped, withdrawal symptoms occur due to the body's need to readjust from the pharmacologically induced imbalance. However, tolerance and withdrawal symptoms do not...
467
Ligand-Gated Ion Channel Receptor: Gating Mechanism
2.2K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.2K
Opioid Receptors: Overview
761
Opioid receptors, including the mu (μ, MOR), delta (δ, DOR), and kappa (κ, KOR) types, belong to the rhodopsin family of G protein-coupled receptors. These receptors are located throughout the central and peripheral nervous systems and in non-neuronal tissues such as macrophages and astrocytes. Opioid receptor ligands can be categorized into agonists or antagonists. Highly selective agonists include [d-Ala2, MePhe4, Gly(ol)5]-enkephalin or DAMGO for MOR, [D-Pen2,...
761
Overview of Cell-Cell Junctions
25.5K
The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another cell. These cell junctions are classified into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight...
Occluding or Tight...
25.5K


