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增加了光学组织的多重性基于清除的瘤微环境的三维免疫光显微镜通过发光二极管光漂白

Jingtian Zheng¹, Yi-Chien Wu¹, Evan H Phillips¹

¹Department of Pharmaceutical Sciences, University of Illinois, Chicago, Chicago, Illinois.

Laboratory investigation; a journal of technical methods and pathology

|April 28, 2024

概括

一种新的发光二极管 (LED) 光漂白方法增强了用于绘制瘤微环境 (TME) 的3D免疫光显微镜. 这种技术可以进行8倍位成像,以3D显示详细的细胞和组织结构.

科学领域:

生物医学成像技术生物医学成像技术
光学显微镜的使用方法
癌症研究癌症研究

背景情况:

三维 (3D) 免疫光 (IF) 显微镜对于研究瘤微环境 (TME) 是至关重要的.
当前的3D IF方法具有有限的多重性,限制了TME组件的同时可视化.
局限性阻碍了对瘤异质性和空间关系的全面分析.

研究的目的:

开发一种先进的方法,用于高复杂度的TME的3D光学映射.
为了克服现有的3D IF显微镜技术的复合限制.
为了能够对TME进行详细的空间分析,用于癌症研究和治疗评估.

主要方法:

开发了一种高功率的LED光漂白装置和温度控制室.
建立循环工作流程:IF染色,组织清除,3D共聚焦显微镜和LED光漂白.
实施组织安装和区域跟踪以进行图像记录和从400微米厚的瘤截面生成8倍位数据.

主要成果:

在不损害组织或抗原完整性的情况下,成功实施LED光漂白.
生成8倍数3D图像数据,可视化TME内的多种血管,免疫和癌细胞.
对免疫治疗后细胞重塑的定量3D空间分析方法的验证.

结论:

开发的LED光漂白系统在3DIF显微镜中显著增加了复合功率.
这种新的方法促进了TME的全面的3D光学映射.
该方法为研究瘤异质性和对治疗的反应提供了新的途径.

关键词:

3D多重显微镜的显微镜.癌症和免疫生物标志物癌症免疫疗法免疫疗法循环的3D免疫光.影像成像技术影像成像技术瘤微环境是一个微环境.

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