在体外对放射性标记的二通过人体皮肤皮肤的皮肤吸收进行比较研究
Sadaff Ejaz1, Clive Roper2, Zoe Finlayson3
1Kenvue Inc., 199 Grandview Rd, Skillman, NJ 08558, USA.
概括
八烯防晒中的杂质索在防晒配方中显示出显著的挥发性. 这种波动导致了低质量平衡,这表明在防晒安全性评估中,皮肤吸收的潜在估计过高.
科学领域:
- 皮肤毒理学和化品科学.
背景情况:
- 八烯是防晒中广泛使用的紫外线过器.
- 二是含有八烯的防晒配方中常见的杂质.
- 了解杂质的皮肤命运对于防晒的安全性至关重要.
研究的目的:
- 调查商业防晒配方中的索的皮肤传递和质量平衡.
- 评估索挥发性对皮肤吸收研究的影响.
- 建议对二进行保守的皮肤吸收值.
主要方法:
- 标有放射性标记的 [14C] -Benzophenone 被添加到两个气溶防晒配方和一个乙载体中.
- 配方应用于静态扩散细胞中的ex vivo人体皮肤.
- 用24小时的液体闪计数分析样本;使用纸评估挥发性.
主要成果:
- 在各种配方中, [14C] - 二的皮肤传递率从5.19%到10.02%不等.
- 低质量余额 (8.02%至85.3%) 表明 [14C] - 二的显著损失.
- 挥发性测试证实了由于蒸发而导致的显著 [14C] - 色损失,质量平衡在24小时内下降到56.9-60.6%.
结论:
- 索具有显著的挥发性,在体外皮肤吸收研究中影响质量平衡.
- 由于挥发性的观察到的损失可能导致实际皮肤吸收的高估.
- 建议保守的皮肤吸收值为12.42%,以考虑到防晒配方中的索挥发性.
相关概念视频
01:16Methods for Studying Drug Absorption: In vitro
233
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
233
01:09Methods for Studying Drug Absorption: In situ
230
In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
230