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Updated: Jun 27, 2025

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
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Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

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使用生物发光共振转移能量的糖蛋白-糖蛋白受体结合检测.

Kamila Adamczuk1, Thu Ha Ngo1, Jakub Czapiński1

  • 1Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland.

Endocrinology
|April 28, 2024
PubMed
概括

我们开发了一种新的生物发光试验,用于研究活细胞中的激素受体相互作用. 这种方法有效地表征了糖蛋白受体及其连接体中的突变,为传统测定提供了安全和具有成本效益的替代方案.

关键词:
高西亚光酶 (Gluc) 是一种光酶.与G蛋白结合的受体 (GPCR)生物发光共振能量转移 (BRET)增强绿色光蛋白 (eGFP) 是一种绿色光蛋白.葡萄糖蛋白质 (glycoproteins) 是一种葡萄糖蛋白质.带结合测定试验黄素化激素/胆性淋巴激素受体 (LHCGR) 的使用.突变是发生在突变中的突变.

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