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Updated: Jun 27, 2025

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使用渐变阻抗光学显微镜进行定量相位成像

Jinming Zhang1, Mirsaeid Sarollahi1, Shirley Luckhart2,3

  • 1Department of Physics, University of Idaho, 875 Perimeter Drive, Moscow, ID, 83844, USA.

Scientific reports
|April 28, 2024
PubMed
概括

梯度延迟光学显微镜 (GROM) 能够对薄和厚的生物样本进行定量相位成像 (QPI). 这项创新增强了在散射环境中的成像对比度和细胞分析.

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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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