患有不同病态形态非小细胞肺癌的患者液细菌微生物组的组成
V G Druzhinin1, E D Baranova2, P S Demenkov3
1Kemerovo State University, Kemerovo, Russia Kemerovo State Medical University, Kemerovo, Russia.
Vavilovskii zhurnal genetiki i selektsii
|April 29, 2024
概括
肺癌微生物组在状细胞癌和腺癌患者之间有所不同. 在状细胞癌中,特定的细菌,如链球菌和细菌增加,这表明潜在的诊断生物标志物.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 基因组学就是基因组学.
背景情况:
- 呼吸道微生物群影响肺癌的发展.
- 微生物组的改变与慢性炎症和基因组损伤有关.
- 肺癌亚型中的呼吸道微生物组组成仍未得到充分研究.
研究的目的:
- 为了研究不同组织类型的肺癌患者唾液微生物组的分类组成.
- 将状细胞癌和腺癌患者的呼吸道微生物组与健康对照组进行比较.
主要方法:
- 16S rRNA 基因 V3-V4 区域的下一代测序 (NGS).
- 分析了52名平细胞癌患者,52名腺癌患者和52名健康对照患者的肠微生物组.
- 使用了Shannon和Pielou指数,Bray-Curtis不相似性和LEFse分析.
主要成果:
- 两组之间物种丰富度 (香农指数) 没有显著差异.
- 在患者和对照组之间,均度 (Pielou指数) 的显著差异.
- 在状细胞癌和对照组之间观察到不同的细菌群体结构 (β多样性),但在腺癌和对照组中没有.
- 与腺癌相比,状细胞癌中Bacillota (Streptococcus,Bacillus) 和Actinomycetota (Rothia) 的丰度增加.
- 在状细胞癌患者和对照患者中,Streptococcus,Bacillus,Peptostreptococcus,Prevotella,Macellibacteroides,Rothia和Actinobacillus的水平增加.
结论:
- 唾液细菌微生物群在非小细胞肺癌的组织学类型之间存在显著差异.
- 特定的细菌种群可以作为肺癌诊断的潜在生物标志物.
- 建议使用全基因组测序进行进一步的研究,以确定细菌物种级生物标志物.
相关概念视频
01:26Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
194
Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
194
01:20Sputum Studies II: Culture and Sensitivity
308
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
308