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在早期发病的结直肠癌中识别差异表达基因和拼接事件

Olivia M Marx^1,2, Marc M Mankarious¹, Walter A Koltun¹

¹Koltun and Yochum Laboratory, Department of Surgery, Division of Colon & Rectal Surgery, Pennsylvania State University College of Medicine, Hershey, PA, United States.

Frontiers in oncology

|April 29, 2024

概括

早期结直肠癌 (EOCRC) 转录组显示出不同的分子特征. 八个基因的签名预测了生存率,为年轻患者提供了新的治疗方法和查的见解.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

结肠直肠癌 (CRC) 发病率在年轻人中正在上升,原因尚不清楚.
了解早期CRC (EOCRC) 的分子差异至关重要.

研究的目的:

在EOCRC中识别不同的基因表达特征 (转录组) 与晚期CRC (LOCRC) 相比.
为了揭示分子和遗传特征区分EOCRC.

主要方法:

在患者匹配的瘤和来自EOCRC (n=21) 和LOCRC (n=22) 队列的正常结肠组织上进行RNA测序 (RNA-seq).
生物信息分析以确定EOCRC特定的基因,通路,细胞群,拼接事件和新抗原.
使用癌症基因组图集结肠腺癌 (TCGA-COAD) 数据库进行验证.

主要成果:

在EOCRC中发现了一个八个基因特征 (ALDOB,FBXL16,IL1RN,MSLN,RAC3,SLC38A11,WBSCR27,WNT11) 预测CRC总体存活率.
在EOCRC.中预测了免疫和非免疫细胞群的不同丰度.
在EOCRC中发现了拼接机械的表达增加和特定的替代拼接事件,包括HOTAIRM1,在EOCRC中发现.
确定了七个EOCRC特有的替代拼接事件,编码潜在的新抗原.

结论:

转录组分析揭示了EOCRC中独特的遗传和分子特征.
结果可能指导未来的CRC查,预后指标和新的治疗目标.

关键词:

结肠直肠癌是什么意思这是早期发病的.晚期开始的晚期开始的拼接拼接拼接拼接转录组 (transcriptome) 是一个转录组.

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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