来自子宫液的外体细胞促进人类精子的电容化
Renbin Deng1, Zhao Wu2, Chaoyong He1
1Department of Urology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
PeerJ
|April 29, 2024
概括
子宫液中的细胞外囊泡 (EV) 增强了精子的功能. 这些EVs促进精子电容,由增加的体反应和反应性氧物种 (ROS) 水平表明.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 细胞外囊泡 (EVs) 对于细胞间通信至关重要,在各种体液中发现.
- 众所周知,来自人类子宫内膜上皮细胞 (EECs) 的EV可以改善精子受精.
- 直接从子宫冲洗液到精子上的EVs的生理作用仍然未被探索.
研究的目的:
- 研究从子宫冲洗液中分离的EVs对人类精子参数的影响.
- 为了确定子宫液EV是否影响精子电容,运动性和反应性氧物种 (ROS) 生产.
主要方法:
- 从子宫冲洗液中隔离EVs.
- 使用纳米粒子追踪分析 (NTA),西部斑 (CD9,CD63) 和传输电子显微镜 (TEM) 进行电动汽车的表征.
- 用EVs化人类精子,并在2h和4h时评估体反应,运动性和ROS水平.
主要成果:
- TEM证实了杯状囊泡,NTA显示大小范围为30-200纳米,Western blot检测了EV标志物CD9和CD63.
- 在化后4小时观察到精子运动的显著差异.
- EV化显著促进了体反应,并增加了精子中的ROS水平.
结论:
- EVs存在于子宫冲洗液中,并具有细胞外囊泡的特征.
- 用子宫液EVs化显著增强人类精子体反应和ROS生产.
- 这些发现表明,子宫液中的EVs在通过子宫过渡期间促进人类精子容量的生理作用.
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