高度单克隆抗体的粘弹性使用粒子追踪微观学
Conor M Lewis1, Charles T Heise2, Natalia Harasimiuk2
1Biological Physics, Department of Physics and Astronomy, University of Manchester, Manchester M13 9PL, United Kingdom.
APL bioengineering
|April 29, 2024
概括
高度单克隆抗体 (mAbs) 具有显著的粘弹性,对于治疗开发至关重要. 它们的粘度随着度的增加而呈指数级增加,影响了生产和药物输送.
科学领域:
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 单克隆抗体 (mAbs) 是关键的治疗药物,高度可以提高疗效和生产效率.
- 了解缩的mAb溶液的物理性质对于优化它们在治疗和制造中的使用至关重要.
- mAbs 的粘性弹性会影响其在生产,配方和药物输送过程中的行为.
研究的目的:
- 研究缩的人性化单克隆抗体 (mAb) 溶液的粘弹性特性.
- 为了确定mAb度和溶液粘度之间的关系.
- 在不同的pH条件下探索mAbs的凝行为.
主要方法:
- 采用高速粒子跟踪微风学来探测mAb溶液.
- 研究了两个具有不同同电点的人性化mAbs.
- 研究了pH对mAb凝的影响.
主要成果:
- 在40 mg/ml以上的mAb溶液中观察到显著的粘性弹性.
- 对于缩的mAb悬浮液,在探测的时间尺度上,功率规律粘弹性是明显的.
- 终端粘度显示出对mAb度的指数依赖,与充电的体系统相一致.
- 降低pH值后的凝过渡跟随功率定律缩放,异常扩散指数与凝时间相反相关.
结论:
- 缩的mAb溶液表现出复杂的粘弹性行为,对其应用至关重要.
- 粘度的度依赖性可以通过修改的穆尼关系来描述.
- mAb凝是一种依赖pH值的现象,具有功率定律缩放特征.
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