相关实验视频

Updated: Jun 27, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

三种与液态相分离相关的基因与结质瘤的预后有关

Ling Lv¹, Xin Zhang¹, Yajun Liu¹

¹Department of Neurosurgery, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, People's Republic of China.

Pharmacogenomics and personalized medicine

|April 29, 2024

概括

失调的液体液相分离 (LLPS) 基因与质瘤有关. 一个新的三基因模型 (TAGLN2,NTNG2,IGF2BP2) 准确地预测了质瘤患者的存活率,并可能指导免疫治疗.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

液-液相分离 (LLPS) 功能障碍与瘤发生有关.
与LLPS相关的基因在质瘤发育和进展中的作用尚不清楚.

研究的目的:

调查与LLPS相关的基因与质瘤之间的关联.
根据LLPS基因表达来识别潜在的质瘤分子亚型.
使用LLPS相关基因开发质瘤的预后模型.

主要方法:

从GSE50161数据集中对质瘤中差异表达基因 (DEGs) 的分析.
使用公共数据库识别和分析LLPS相关基因.
使用TCGA-glioma数据集进行质瘤分子亚型的确定.
使用CIBERSORT进行免疫细胞透分析.
使用考克斯回归和RT-qPCR的预后模型的开发和验证.

主要成果:

在质瘤中发现了673个具有差异表达的LLPS相关基因.
确定了三种不同的质瘤分子亚型 (C1,C2,C3),显示PD1/PDL1表达和免疫细胞透的变化.
开发了三基因预后特征 (TAGLN2,NTNG2,IGF2BP2),表明风险组之间的生存率有显著差异.
预后模型显示1,3年和5年生存期的高精度 (AUC值).
在质瘤组织和细胞中,TAGLN2和IGF2BP2被上调,而NTNG2被下调.

结论:

与LLPS相关的基因在质瘤分级和预后方面发挥着重要作用.
开发的三基因预后模型为改善质瘤个性化预后和免疫治疗策略提供了潜力.

关键词:

质瘤质瘤是一种免疫细胞的透.液态-液态相隔离方法液态相隔离方法分子亚型分子亚型预测模型的预测模型.

更多相关视频

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

The Retinoblastoma Gene

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...