对Helicobacter pylori的诊断方法:在胃活检和愿望中检测的比较研究
Radha Kumari1, Manoj Kumar1, Kumari Seema1
1Microbiology, Rajendra Institute of Medical Sciences (RIMS), Ranchi, IND.
Cureus
|April 29, 2024
概括
快速尿酶试验 (RUT) 对于检测活检组织中的Helicobacter pylori具有高度敏感性. 聚合酶连锁反应 (PCR) 在胃吸附物中提供了更高的灵敏度,活检组织是整体最佳样本.
科学领域:
- 医学微生物学 医学微生物学
- 胃肠病学 胃肠病学
- 诊断病理学的诊断病理学
背景情况:
- 杆菌 (Helicobacter pylori) 是一种流行的人类病原体,与胃部疾病有关.
- 它是一种微气友细菌,具有单极鞭毛体的特征.
- 感染与慢性胃炎,,腺癌和淋巴瘤有关.
研究的目的:
- 为了比较快速尿酶试验 (RUT) 和聚合酶连锁反应 (PCR) 检测H. pylori的有效性.
- 用胃活检和吸血样本评估RUT和PCR的敏感性和特异性.
- 为了确定*H. pylori*诊断的最佳样品类型.
主要方法:
- 从110名患者中采集了内镜活检和胃吸血样本.
- 用RUT和PCR分析了样本.
- 对每个方法和样本类型计算了灵敏度,特异性,PPV,NPV和精度.
主要成果:
- RUT在活检组织 (97.18%) 中表现出更高的灵敏度,而不是胃吸附物 (78.94%).
- PCR的敏感性和特异性分别为93.33%和90.0%.
- 与胃吸液相比,活检组织被证明是*H. pylori*检测的优越标本.
结论:
- RUT是一种快速而准确的侵入性测试,用于检测活检组织中的*H. pylori*.
- 在胃吸血中检测*H. pylori*时,PCR更敏感.
- 胃活检组织是可靠*H. pylori*诊断的首选样本.
相关概念视频
01:28Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies
115
Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
115
01:16Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
379
Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
379
01:30Serum Laboratory Studies, Stool Test, Breath Test
342
Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
342
01:23Gastritis III: Clinical Manifestations and Management
232
The clinical manifestations of gastritis can vary depending on the cause and type of gastritis, but some common symptoms may include the following.
Clinical manifestations of acute gastritis
The patient with acute gastritis may have a rapid onset of symptoms, such as epigastric pain or discomfort, dyspepsia, anorexia, hiccups, or nausea and vomiting, which can last from a few hours to a few days. Erosive or hemorrhagic gastritis may cause bleeding, which may manifest as blood in vomit or as...
Clinical manifestations of acute gastritis
The patient with acute gastritis may have a rapid onset of symptoms, such as epigastric pain or discomfort, dyspepsia, anorexia, hiccups, or nausea and vomiting, which can last from a few hours to a few days. Erosive or hemorrhagic gastritis may cause bleeding, which may manifest as blood in vomit or as...
232
01:26Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
194
Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
194
01:26Peptic Ulcer Disease IV: Management
89
Medical treatment strategies for peptic ulcers encompass various methods. The primary goal of treatment is to diminish gastric acidity and strengthen mucosal defense mechanisms.
The therapeutic approach involves ensuring adequate rest, implementing drug therapy, promoting smoking cessation, making dietary modifications, and emphasizing long-term follow-up care.
Pharmacological management
The prevailing therapy for peptic ulcers involves a combination of managing the patient's current...
The therapeutic approach involves ensuring adequate rest, implementing drug therapy, promoting smoking cessation, making dietary modifications, and emphasizing long-term follow-up care.
Pharmacological management
The prevailing therapy for peptic ulcers involves a combination of managing the patient's current...
89