大麻 (Cannabis sativa L.) 蛋白质:提取方法和品种对结构,功能和营养质量的影响
Laura Eckhardt1, Fan Bu1, Adam Franczyk2
1Department of Food Science and Nutrition, University of Minnesota, Saint Paul, MN, 55108, USA.
Current research in food science
|April 29, 2024
概括
提取麻蛋白分离物 (HPI) 的两种方法产生了高纯度和产量. 虽然在中性pH下溶解度很差,但HPI在酸性条件下表现良好,对食品应用有很大的希望.
科学领域:
- 食品科学 食品科学 食品科学
- 植物性蛋白质 植物性蛋白质
- 可持续的食物来源 可持续的食物来源
背景情况:
- 大麻 (Cannabis sativa L.) 是一种新兴的植物性蛋白质提取的可持续来源.
- 开发生产高纯度麻蛋白分离物 (HPI) 的高效方法对于食品应用至关重要.
研究的目的:
- 为了评估性提取与异电沉 (AE-IEP) 和盐提取与超 (SE-UF) 结合的HPI生产.
- 根据提取方法和品种来描述HPI的结构特性,功能性能和营养质量.
- 将结构特征与功能和营养结果联系起来.
主要方法:
- 麻蛋白分离物 (HPI) 是使用AE-IEP和SE-UF方法生产的.
- 进行了结构特征,包括表面疏水性和电荷.
- 评估了可溶性和凝强度等功能性质,并与商业大豆和豆蛋白分离物 (cSPI,cPPI) 相比较.
主要成果:
- 无论是AE-IEP还是SE-UF方法都实现了高HPI纯度 (86.6-88.1%) 和产量 (81.6-87.3%).
- 在中性pH下HPI的溶解性很差,但在酸性pH下溶解性很好 (50-67%),凝强度与cSPI相当.
- AE-IEP产生的HPI功能略好于SE-UF,在四种大麻品种中观察到的差异很小.
结论:
- 使用AE-IEP和SE-UF方法可以实现有效的HPI提取.
- HPI的功能性质,特别是酸性pH和凝强度的溶解性,使其适用于某些食品应用.
- 进一步的研究可以优化提取过程,并指导繁殖计划,以提高大麻蛋白质的特性.
相关概念视频
Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
Protein and Protein Structures
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
Protein Denaturation
The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...


