全转录基因组测序识别了一种具有竞争力的内源RNA网络,该网络调节了膀癌的免疫力
Sanhe Liu1,2,3, Yiqi Wang1,4, Liqun Duan5,1
1College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, 430070, China.
Heliyon
|April 29, 2024
概括
这项研究揭示了一个具有竞争力的内源性RNA (ceRNA) 网络,涉及膀癌中的非编码RNA. 鉴定到的网络,包括BDNF和miR-107,可能成为新的预后标记物和膀癌的治疗点.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 基因组学就是基因组学.
背景情况:
- 非编码RNAs,包括circRNAs,lncRNAs和miRNAs,通过竞争性内源RNA (ceRNA) 网络调节基因表达.
- 在膀癌免疫微环境中,ceRNA网络的作用仍未得到充分研究.
研究的目的:
- 在膀癌的免疫微环境中研究ceRNA调节网络的功能.
- 确定膀癌的潜在预后标志物和治疗点.
主要方法:
- 在38名患者的膀瘤和周围瘤组织上进行了全转录组测序.
- 差异表达的circ-, lnc-, mi-, 和mRNA被选,以构建一个circ/lnc-mi-mRNA调节网络.
- 为了验证发现,对7个公共数据集进行了元分析.
主要成果:
- 一个关键的免疫相关基因BDNF被确定并纳入最终的ceRNA网络.
- 该网络涉及hsa-miR-107被hsa-circ-000211,AC108488.1和LINC00163.1所使用.
- 低BDNF表达和高hsa-miR-107表达与更长的患者存活时间相关.
结论:
- 在膀癌中发现了一种新的ceRNA调节网络.
- 这种涉及BDNF和hsa-miR-107的网络显示出作为预后标记物的潜力.
- 已识别的ceRNA网络代表了膀癌的有希望的分子治疗标.
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