CompCorona:一种用于对冠状病毒进行比较转录组分析的网络应用程序揭示了SARS-CoV-2特定宿主反应
Rana Salihoğlu1,2, Fatih Saraçoğlu3, Mustafa Sibai4
1Department of Bioinformatics, University of Würzburg, Würzburg, Germany.
Turkish journal of biology = Turk biyoloji dergisi
|April 29, 2024
概括
SARS-CoV-2 感染独特地降低了肺细胞中的关键基因的调节,可能导致上皮质功能障碍. 我们的新工具CompCorona有助于研究冠状病毒疾病机制和长期COVID.
科学领域:
- 病毒学和转录学.
- 免疫学和宿主-病原体相互作用
- 计算生物学和生物信息学
背景情况:
- 了解宿主对SARS-CoV-2的转录组反应对于解决长期COVID和相关炎症状况至关重要.
- 调查冠状病毒家族感染的共同和独特机制对于了解疾病进展至关重要.
研究的目的:
- 分析和比较宿主对SARS-CoV-2,SARS-CoV和MERS-CoV感染的转录组反应.
- 开发一个交互式工具 (CompCorona) 用于可视化和分析病毒转录组数据.
- 为了确定与SARS-CoV-2感染相关的特定宿主反应模式.
主要方法:
- 对感染SARS-CoV-2,SARS-CoV和MERS-CoV的肺和外周血液单核细胞 (PBMC) 的全面转录组和丰富分析.
- 开发和使用CompCorona,这是一个用于2D/3D主要组件分析 (PCA) 和路径分析的在线工具.
- 预处理数据集的公开发布以进行比较分析.
主要成果:
- 在肺细胞中,SARS-CoV-2感染独特地降低了血管新生素 (ANG) 和血管内皮生长因子A (VEGFA),表明潜在的上皮和血管功能障碍.
- 在SARS-CoV-2感染细胞中观察到的TNF信号的抑制和补充/凝固通路的激活.
- 在所有三种测试的冠状病毒中,核糖体通路抑制是常见的发现.
结论:
- SARS-CoV-2 感染可能会诱导肺上皮功能障碍,与其他 CoVs 不同.
- CompCorona工具和相关数据集为研究Cov-associated综合征和长期COVID提供了宝贵的资源.
- 进行比较的转录基因分析揭示了对SARS-CoV-2感染的特定宿主反应.
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