循环中的外体CircRNAs作为急性心肌梗塞诊断生物标志物
Xiaoyan Liu1,2,3, Yeping Zhang1,2, Wen Yuan3
1Heart Center and Beijing Key Laboratory of Hypertension, Beijing Chao-Yang Hospital, Capital Medical University, 100020 Beijing, China.
Frontiers in bioscience (Landmark edition)
|April 29, 2024
概括
外体中的循环RNAs (circRNAs) 在急性心肌梗塞 (AMI) 患者中显示出改变的表达. circRNA hsa_circ_0001558是诊断AMI的一个有前途的生物标志物,使其与非心脏性胸痛区分开来.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 心血管研究研究心血管研究
背景情况:
- 外体循环RNAs (circRNAs) 对急性心肌梗塞 (AMI) 的诊断实用性仍未得到充分研究.
- 现有的研究表明,circRNAs在心血管疾病中的作用.
研究的目的:
- 调查外体环RNAs作为AMI生物标志物的诊断潜力.
- 为了确定在AMI患者中差异表达的特定circRNAs.
主要方法:
- 高通量测序被用来识别AMI患者的外体内差异表达的circRNAs.
- 实时聚合酶连锁反应 (RT-PCR) 用于验证选定的circRNAs的表达.
- 该研究包括120名AMI患者和83名非心脏性胸痛患者 (NCCP).
主要成果:
- 测序揭示了AMI患者中893个差异表达的外体环RNA (118个上调,775个下调).
- 三种circRNAs (hsa_circ_0001558,hsa_circ_0001535,hsa_circ_0000972) 在AMI患者中显著上调,AUC值从0.683到0.79.
- 与NCCP相比,hsa_circ_0001558显示AMI患者增加了4.45倍 (AUC = 0.793),ST升高心肌梗塞 (STEMI) 和非ST升高心肌梗塞 (NSTEMI) 的表达更高.
结论:
- 在AMI和NCCP患者之间,外体环RNA表达存在显著差异.
- hsa_circ_0001558被确定为AMI的潜在诊断生物标志物.
- 需要进一步的研究,以充分确定外体circRNAs在AMI诊断中的临床实用性.
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