负压伤口治疗中的一个新机制:介质蛋白-17改变了染色体可访问性概况
Shuao Xiao1, Wenxuan Wang2, Congying Zhao1
1Department of Plastic and Burn Surgery, Second Affiliated Hospital of Air Force Medical University, Xi'an, People's Republic of China.
American journal of physiology. Cell physiology
|April 29, 2024
概括
负压伤口治疗 (NPWT) 通过上调IL-17,一个关键的信号通路,增强伤口愈合. 这项研究揭示了NPWT改变了色素的可访问性,为其在伤口修复中的治疗作用提供了一个新的机制.
科学领域:
- 伤口治愈研究研究 伤口治愈研究
- 分子生物学分子生物学
- 生物医学工程 生物医学工程
背景情况:
- 负压伤口治疗 (NPWT) 是一种广泛使用的临床治疗方法,用于增强伤口愈合.
- 在NPWT的有效性背后的精确分子机制仍然不完全理解.
- 研究这些机制对于优化治疗策略至关重要.
研究的目的:
- 阐明NPWT促进伤口愈合的分子机制.
- 研究IL-17信号通路在NPWT介导的伤口修复中的作用.
- 探索NPWT对皮肤纤维细胞中的染色质可访问性的影响.
主要方法:
- 建立使用NPWT应用的老鼠皮肤伤口愈合模型.
- 维门丁,原I和MMP9.9的免疫光 (IF) 检测结果
- 基因和蛋白质表达和染色质可访问性分析的RNA-sequencing (RNA-seq),西部涂抹,定量PCR和ATAC-seq.
主要成果:
- 在早期的伤口愈合中,NPWT显著促进了重新表皮化,细胞外基质 (ECM) 合成和增殖.
- 差异表达的基因在IL-17信号通路中被丰富,IL-17被确定为由NPWT上调调节的.
- 使用secukinumab (SEC) 抑制IL-17消除了NPWT的有益作用;NPWT和IL-17改变了纤维细胞中的染色质可访问性.
结论:
- NPWT可以调节IL-17以促进伤口愈合,这是一种新的机制.
- NPWT和IL-17刺激改变了皮肤纤维细胞中的染色质可访问性.
- 这项研究为NPWT在伤口修复中的有效性的分子基础提供了新的见解.
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