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缺乏ER局部化过氧素Pex23或Pex29的汉森菌多态细胞显示线粒体功能和形态的缺陷

Haiqiong Chen¹, Rinse de Boer¹, Arjen M Krikken¹

¹Molecular Cell Biology - Groningen Biomolecular Sciences and Biotechnology Institute, Nijenborgh 7, 9747 AG Groningen, The Netherlands.

|April 29, 2024

概括

在Hansenula polymorpha中,Pex23家族蛋白质对于过氧体和脂质滴滴的形成至关重要. 它们的缺失会影响线粒体的结构和功能,影响细胞生长.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
酵母遗传学酵母遗传学

背景情况:

佩克斯23家族蛋白质是参与过氧体和脂质体生物发生的内质网膜局部化因子.
汉塞努拉多态 (Hansenula polymorpha) 拥有四个Pex23家族成员:Pex23,Pex24,Pex29和Pex32. 这四个成员分别是Pex23,Pex24,Pex29和Pex32.
之前的研究表明,Pex24和Pex32是通过Peroxisome-endoplasmic网膜接触部位形成Peroxisome的重要组成部分.

研究的目的:

为了研究所有四种Pex23家族蛋白质缺失对Hansenula polymorpha.细胞器官的影响.
确定单个Pex23家族成员在过氧体,脂质滴和线粒体动态中的特定作用.
阐明Pex23家族蛋白质与线粒体-内分泌网膜相互作用之间的关系.

主要方法:

在Hansenula polymorpha中为Pex23,Pex24,Pex29和Pex32生成和分析单一删除菌株.
细胞器官的显微镜检查,包括真空孔,脂质滴和线粒体,在删除突变.
基因操纵,包括删除DNM1,以评估观察到的表型的抑制.
分析线粒体活动和细胞生长率.

主要成果:

在所有删除菌株中,真空管没有受到影响.
缺少Pex23或Pex29,但没有Pex24或Pex32,导致脂质滴滴数量减少.
线粒体被碎片化并聚集在pex23和pex29细胞中,与Fzo1水平降低和线粒体功能受损有关.
删除DNM1抑制了线粒体形态缺陷,并在pex23和pex29突变物中部分挽救了生长迟缓.
一个人工的线粒体-内等离子体网膜连接部分抑制了生长缺陷,表明有机体接触点发生了变化.

结论:

不同的Pex23家族成员调节器官生物发生的不同方面,Pex23和Pex29特别影响脂质滴形成.
佩克斯23和佩克斯29对于维护线粒体的完整性和功能至关重要,可能通过调节线粒体-内分泌网膜接触部位.
Pex23家族在细胞组织中发挥着多方面的作用,超越过氧化体,影响脂质滴和线粒体.

关键词:

脂质滴滴是指脂质的滴滴.线粒体中的线粒体.佩克斯23家族的家庭成员.酵母酵母是一种酵母.

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