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来自Cutibacterium acnes的细胞外囊泡在细胞癌中促进瘤生长

Kentaro Jingushi¹, Atsunari Kawashima², Sayaka Tanikawa¹

¹Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.

|April 29, 2024

概括

血清细胞外囊中的Cutibacterium acnes DNA与细胞癌有关. 这种在瘤组织中发现的细菌DNA促进癌症生长和血管生成,这表明一种新的瘤促进机制.

科学领域:

微生物学微生物学
在瘤学瘤学.
细胞外囊泡生物学细胞外囊泡生物学

背景情况:

细菌菌群与各种疾病有关,但它们在以前被认为是无菌的血液中的存在越来越被认可.
已经在血液中检测到细菌16S rRNA基因,具有与疾病相关的特征性微生物组,但起源和机制仍然不清楚.

研究的目的:

在细胞癌 (RCC) 患者的血清细胞外囊泡 (EV) 中研究细菌DNA的存在和作用.
确定血清EVs中的Cutibacterium acnes (C. acnes) 是否与RCC相关,并有助于瘤进展.

主要方法:

在来自健康捐赠者和RCC患者的血清EV中对细菌DNA的16SrRNA元基因组分析.
在手术前和手术后血清EV,瘤组织和相关微生物群中检测C. acnesDNA.
在体外评估C. acnes EV被癌细胞吸收.
在小鼠模型中对C. acnes EV瘤促进活性进行体内评估.

主要成果:

在RCC患者的血清EV中,Cutibacterium acnes DNA被确定为一种特征性的细菌DNA.
与手术前相比,手术后血清EVs中的C. acnesDNA水平显著降低.
在瘤组织和相关微生物群中发现了C. acnes DNA,这表明局部化.
在小鼠模型中,C. acnes EVs增强了癌细胞的扩散,并促进了血管生成.

结论:

由C. acnes在细胞癌组织中释放的细胞外囊泡与这种疾病有关.
C. acnes EVs具有促进瘤的活性,增强癌细胞的增殖和血管生成.
这项研究表明,C. acnes EVs有助于细胞癌的进展.

关键词:

这种细菌是Cutibacterium acnes.16S rRNA 基因是16S 的基因.细菌的 DNA 是细菌的 DNA.细胞外囊泡中的细胞外囊泡.细胞癌瘤是细胞癌.

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