一个背部皮肤折叠窗口腔瘤小鼠模型用于结合肠道显微镜和磁共振成像在转化癌症研究
W Jeffrey Zabel1, Nader Allam2, Hector Alejandro Contreras Sanchez2
1Department of Medical Biophysics, University of Toronto; jeff.zabel@mail.utoronto.ca.
Journal of visualized experiments : JoVE
|April 29, 2024
概括
这项研究引入了一种用于癌症研究的新型小鼠模型,将高分辨率临床前成像与CT和MRI等临床模式相结合. 这允许直接关联发现和验证新的成像技术.
科学领域:
- 在瘤学瘤学.
- 医疗成像医学成像
- 翻译研究是翻译研究.
背景情况:
- 临床前的内脏成像提供了高分辨率但有限的组织透.
- 临床成像 (CT,MRI,PET) 具有深度透,但分辨率较低.
- 在将微型临床前发现转化为宏观临床应用方面存在差距.
研究的目的:
- 开发一种新的背部皮肤折叠窗口腔瘤小鼠模型.
- 为了使同一个动物能够同时进行临床前的静脉内科和临床成像 (CT,MR).
- 建立一个图像分析平台,共同注册和关联多式联络成像数据.
主要方法:
- 使用背部皮肤折叠窗口腔瘤小鼠模型.
- 综合性临床前静脉内显微镜与临床CT和MR成像.
- 采用信托标记器用于3D共注册成像模式.
- 开发了一个用于数据集成的图像分析平台.
主要成果:
- 证明了在同一动物中同时注册高分辨率的静脉内视图和低分辨率的临床成像的可行性.
- 启用了"地面真相"显微镜发现和临床成像数据之间的直接空间一致性.
- 促进了对瘤对各种疗法的反应的纵向监测.
结论:
- 描述的窗口腔模型弥合了癌症成像中的微观至宏观分辨率差距.
- 这个平台对于验证现有的临床成像方法和开发新的方法是有价值的.
- 它有助于将临床前静脉内显微镜发现转化为临床上适用的成像模式.
更多相关视频
08:52Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
Published on: January 19, 2018
13.9K
10:25Author Spotlight: Integrating High-Resolution Intravital Imaging and MRI to Enhance Stereotactic Body Radiation Therapy Planning
Published on: April 12, 2024
1.4K
相关概念视频
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...