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环境DNA隔离,验证和保存方法

Seth W Cunningham^1,2, Michael Tessler^3,4,5, Jicayla Johnson-Rosemond^6,7

¹Department of Biological Sciences, Fordham University, Bronx, NY, USA. scunningham@amnh.org.

Methods in molecular biology (Clifton, N.J.)

|April 29, 2024

概括

这项研究详细介绍了环境DNA (eDNA) 隔离,验证和保存方法. 它提供了一种具有成本效益的协议,用于从多种样本中提取eDNA,强调污染控制和适当的存储,以便将来研究.

科学领域:

分子生物学分子生物学
生态生态学生态生态学
环境科学环境科学

背景情况:

环境DNA (eDNA) 分析依赖于分子技术,但需要专门的工作流程.
污染控制和适当的负控制在整个eDNA过程中至关重要,从收集到保存.

研究的目的:

详细介绍eDNA隔离,验证和保存的基线方法.
为了呈现一个具有成本效益的,家庭制造的协议,优化了eDNA提取.

主要方法:

收集和保存各种电子DNA样本 (土壤,水,空气,组织).
样品处理包括抑制剂去除和物理干扰.
标准DNA分离 (基于套件或::异胺 [PCI]) 和量化 (度仪).
采样材料和提取的DNA的长期储存策略.

主要成果:

建立了用于eDNA隔离,验证和保存的协议.
展示了一种具有成本效益的本地酒提取协议.
强调了在基因放大之前产量量化的重要性.

结论:

标准化方法对于可靠的eDNA分析至关重要.
适当的保存确保了样本的完整性,并使未来的研究成为可能.
一个具有成本效益的协议可以促进更广泛的eDNA应用.

关键词:

DNA定量化 DNA定量化储存 DNA DNA 的存储.核酸隔离的核酸分离方式样品保存样品的保存我们的eDNADNA

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