在Drosophila轴延伸过程中,极化细胞间隙是坚固的,可以通过侵入性中皮体对直角拉动
Claire M Lye1, Guy B Blanchard1, Jenny Evans1
1Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom.
PLoS biology
|April 29, 2024
概括
在Drosophila发育过程中,中皮层的侵入力机械地拉动外皮层,但不会破坏细胞间隙. 然而,它确实减缓了细胞延长,影响了轴延伸.
科学领域:
- 发育生物学是发展生物学.
- 细胞力学 细胞力学
- 形态发生 形态发生 形态发生
背景情况:
- 组织形态发生涉及发育组织之间的机械相互作用.
- 德洛索菲拉的胃化特征是重叠的中皮体内和外皮体生殖带延伸.
- 带扩展依赖于由Myosin II驱动的细胞间隔.
研究的目的:
- 调查中皮层发育对外皮层延伸的机械和机械传导效应.
- 检查对Myosin II招募和细胞间隔的影响.
- 确定细胞与机械力之间的合的强度.
主要方法:
- 在Drosophila胃化过程中研究了中皮和外皮之间的机械合.
- 评估了Myosin II平面极化和连接动态.
- 对细胞延长和邻居交换的量化影响.
主要成果:
- 确认了机械合:中膜拉拉变形ectoderm正向生殖带延伸.
- 没有观察到Myosin II平面极化或细胞间隔率的显著变化.
- 介质皮层的侵入减缓了前后细胞的延长,抵消了内皮层的紧张.
结论:
- 极化细胞间隙,主要轴延伸机制,是强大的 mesoderm invagination 力量.
- 介质皮的侵蚀通过减缓细胞延长而对轴延伸施加反作用力.
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