石英晶微平衡作为一个整体检测器量化复杂有机矩阵在液体染色学: 1. 合,表征和验证的方法
Christopher Wabnitz1, Aoife Canavan1, Wei Chen1
1TUM School of Natural Sciences, Chair of Analytical Chemistry and Water Chemistry, Technical University of Munich, Garching 85748, Germany.
这项研究引入了石英晶微平衡 (QCM) 干质量传感,用于在梯度高性能液态色谱 (HPLC) 清理过程中量化复杂的有机矩阵. 这种方法简化了矩阵量化,有助于优化具有挑战性的HPLC分离过程.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 传感器技术 传感器技术
背景情况:
- 复杂的样本矩阵可以干扰微量有机化合物分析.
- 高性能液态色谱 (HPLC) 对于样品清理至关重要,但由于缺乏实时矩阵检测,优化它是具有挑战性的.
- 在HPLC中的梯度化进一步复杂化了清理过程中的矩阵量化.
研究的目的:
- 探索石英晶体微平衡 (QCM) 干质量传感在梯度HPLC过程中量化复杂有机矩阵的潜力.
- 在HPLC清洁中开发和验证实时矩阵量化系统.
- 为了解决优化HPLC清洁方法的局限性.
主要方法:
- 通过流量分割器将QCM和微流体喷雾干燥器与商业HPLC系统相连.
- 为集成系统制定校准和数据处理策略.
- 描述系统的检测和定量极限,用于各种化物组成.
主要成果:
- 达到4.315 mg/L的检测极限 (LOD) 和1652 mg/L的量化极限 (LOQ).
- 通过对环境样本中的自然有机物质与总有机碳分析进行验证,证明了可行性.
- 对天然有机物质的绝对回收率为103±10%.
结论:
- 在HPLC清理过程中,QCM干质量传感为实时矩阵量化提供了可行的解决方案.
- 开发的系统可以帮助分析师优化HPLC清洁方法,特别是使用梯度溶剂.
- 这种方法对需要高效的样本准备和清理的各种分析领域具有前景.
更多相关视频
07:11Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
Published on: November 1, 2018
09:46Adsorption Device Based on a Langatate Crystal Microbalance for High Temperature High Pressure Gas Adsorption in Zeolite H-ZSM-5
Published on: August 25, 2016
相关概念视频
High-Performance Liquid Chromatography: Types of Detectors
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Gas Chromatography: Overview of Detectors
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Analyzers: Common Types
Gas Chromatography: Types of Detectors-II
