核酸酸酶可以制造N7-松氨酸.
Sarah Westarp1,2, Felix Brandt3, Lena Neumair1
1Chair of Bioprocess Engineering, Institute of Biotechnology, Faculty III Process Sciences, Technische Universität Berlin, Ackerstrasse 76, 13355, Berlin, Germany.
酶通常在特定位置 (N9) 附着核酸与酸基. 然而,核酸酸化酶也会产生一种不同的形式,N7-素,这表明这些酶不是完美的催化剂.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 自然产品生物合成 自然产品生物合成
背景情况:
- 核酸加工酶是高度进化的,具有已建立的区域选择性.
- purin核基的糖化主要发生在 N9 位置.
研究的目的:
- 调查核酸糖化中的N9区域选择性的例外情况.
- 为了表征一种新型的Ribosylation区域异构体的Xanthosine.
主要方法:
- 使用野生型核酸酸化酶的酶分析.
- 对合成的核酸进行光谱分析.
- 确定物理化学性质,包括吸收光谱和解平衡.
主要成果:
- 发现野生型核酸酸化酶产生N7-色素,这是色素的区域异构体.
- 素表现出不典型的特性:红移吸收光谱,高解平衡常数和低酸度.
- 这代表了与预期的N9糖基化反应的偏差.
结论:
- 素的生物合成表明,即使是来自初级代谢的高度进化的酶也可以成为不完美的催化剂.
- 这一发现挑战了核酸加工中完美的区域选择性的范式.
- 一种以前未知的天然产品N7-丁已被确定和描述.
更多相关视频
08:46Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
Published on: July 26, 2018
11:37Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
Published on: July 28, 2017
相关概念视频
Proofreading
Phosphodiester Linkages
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
ATP and Macromolecule Synthesis
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Preparation of Nitriles
Phase II Reactions: Miscellaneous Conjugation Reactions
A key example involves the conjugation of cyanide ions, which impair cellular respiration and alter hemoglobin into non-oxygen-carrying cyanmethemoglobin. To neutralize this threat, a sulfur atom from thiosulphate is transferred to the cyanide ion, catalyzed by the enzyme rhodanese, resulting in an inactive compound called thiocyanate. The production of...
