Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phosphorylation01:02

Phosphorylation

50.3K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
50.3K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

13.1K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
13.1K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Pseudokinase-converting mutation in protein kinase C alpha drives chordoid glioma by pathway rewiring.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Protein kinase C theta: evolution, regulation, and function.

The Biochemical journal·2026
Same author

Sex-specific disruptions in PKCγ signaling in a mouse model of spinocerebellar ataxia type 14.

JCI insight·2026
Same author

How to build the regulatory genome: a constructionist guide to the cis-regulatory code.

Development (Cambridge, England)·2026
Same author

Polygenic backgrounds influence phenotypic consequences of variants in cells, individuals, and populations.

Cell genomics·2026
Same author

RAPID-DASH: fast and efficient assembly of guide RNA arrays for multiplexed CRISPR-Cas9 applications.

Synthetic biology (Oxford, England)·2026

相关实验视频

Updated: Jun 27, 2025

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

9.2K

以后翻译修改为中心的基础编辑器屏幕,以评估高吞吐量酸化站点的功能.

Patrick H Kennedy1,2,3, Amin Alborzian Deh Sheikh1,2,3, Matthew Balakar4

  • 1Laboratory for Immunochemical Circuits, La Jolla Institute for Immunology, La Jolla, CA, USA.

Nature methods
|April 29, 2024
PubMed
概括

科学家们开发了一种新方法来研究数千种动态后翻译性修饰 (PTM) 和它们在基因表达中的作用. 这种方法揭示了以前未知的酸化部位,可以微调细胞信号通路.

更多相关视频

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

18.6K
Oligopeptide Competition Assay for Phosphorylation Site Determination
09:16

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

8.4K

相关实验视频

Last Updated: Jun 27, 2025

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

9.2K
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

18.6K
Oligopeptide Competition Assay for Phosphorylation Site Determination
09:16

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

8.4K

科学领域:

  • 分子生物学分子生物学
  • 细胞信号传输 细胞信号传输
  • 基因组学就是基因组学.

背景情况:

  • 细胞功能是由成千上万的动态后翻译性修改 (PTM) 协调的,但将这些修改与基因电路连接起来的技术在规模上是有限的.
  • 目前对信号通路的理解往往集中在有限数量的关键酸化和转录事件上,忽视了PTM的复杂性.

研究的目的:

  • 开发一种高通量方法,用于评估酸化部位对细胞过程的功能影响.
  • 研究未研究的酸化部位在T细胞激活和转录调节中的作用.

主要方法:

  • 开发以PTM为中心的基础编辑与表型查相结合.
  • 暂时解决的蛋白质组的应用,以指导功能评估.
  • 利用T细胞激活作为研究信号通路的模型系统.

主要成果:

  • 确定了数百个以前未被研究的酸化位,这些位调节激活T细胞核因子 (NFAT) 转录活性.
  • 发现酸化调解PHLPP1的核定位,影响NFAT和核因子kappa B (NFκB) 活动.
  • 证明特定的酸突变可以微妙地改变基因表达模式,表明对微调转录反应的能力.

结论:

  • 基础编辑器对PTM站点的选提供了一个强大的平台,可以在复杂的信号通路中剖析翻译后修改的功能角色.
  • 这些发现突出了许多酸化事件对细胞调节和基因表达的显著,但往往被忽视的贡献.