微生物组元基因组测序中的可变性和偏差:一个实验室间的研究,比较实验协议
Samuel P Forry1, Stephanie L Servetas2, Jason G Kralj2
1Complex Microbial Systems Group, National Institute of Standards and Technology (NIST), Gaithersburg, MD, USA. sam.forry@nist.gov.
Scientific reports
|April 29, 2024
概括
方法选择显著影响微生物组元基因组测序 (MGS) 结果,影响测量偏差和稳定性. 这项研究强调了需要标准化协议,以提高独立微生物组分析的可比性.
科学领域:
- 微生物学 微生物学
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
背景情况:
- 微生物组分析的方法变化使得结果复制和可比性变得复杂.
- 标准化对于可靠和可复制的微生物组研究至关重要.
研究的目的:
- 评估方法选择对微生物组元基因组测序 (MGS) 结果的影响.
- 调查MGS分析的方法格局,并确定影响结果的关键变量.
- 为了评估不同MGS协议的测量偏差和稳定性.
主要方法:
- 一项国际间实验室研究 (马赛克标准挑战 - MSC) 涉及44个实验室.
- 使用参与者的标准实验室方法分析了7个共享参考样本 (人类便和模拟社区).
- 收集关于100个协议变量的详细元数据,用于16S rRNA基因扩增和全基因组测序 (WGS) 数据.
主要成果:
- 协议选择显著影响MGS测量偏差和稳定性.
- 即使在使用类似方法的实验室中也观察到结果的变化.
- 测量偏差在DNA模拟社区分析中很明显,尽管存在普遍共识.
结论:
- 方法决策对微生物组测序数据的可比性和可靠性产生重大影响.
- 标准化MGS协议对于推进微生物组研究至关重要.
- 结果为设计未来微生物组测量研究提供了洞察力.
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