相关实验视频

Updated: Jun 27, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

功能化的纳米线用于miRNA介导的原始T细胞的治疗编程

Kristel J Yee Mon¹, Sungwoong Kim^2,3,4, Zhonghao Dai³

¹Department of Microbiology and Immunology, Cornell University, Ithaca, NY, USA.

Nature nanotechnology

|April 29, 2024

概括

这项研究引入了纳米线,用于编程原始T细胞,而无需预先激活,从而增强了采用T细胞疗法. 这种方法通过调节T细胞种群来改善对病原体的免疫保护.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
纳米技术纳米技术

背景情况:

采用T细胞治疗的有效性受到现有的ex vivoT细胞工程方法的限制,这些方法需要预先激活,从而导致失去原始T细胞状态.
编程原始T细胞对于改善采用T细胞治疗的治疗结果至关重要.

研究的目的:

开发一种方法来预编程初级原始CD8+T细胞,使用纳米线传递微RNA,而无需预激活.
评估纳米线介导的微RNA输送对T细胞分化,健康和体内治疗疗效的影响.

主要方法:

利用基聚合物功能化的纳米线,将单个或多个微RNA (miR-29和miR-130) 传递给原始原始老鼠和人类CD8+ T细胞.
使用纳米导线输送大,整体的lentiviral粒子,以实现潜在的长期集成.
分析了T细胞增殖,表型,转录调节,效应分子分泌和体内免疫保护,对抗流感和Listeria monocytogenes.

主要成果:

通过纳米线介导的miR-29和miR-130的同时递送成功编程了原始CD8+T细胞,调节了T细胞的适应性和分化命运.
编程的T细胞表现出改变的增殖,表型,转录调节和效应分子分泌.
在体内采用纳米电线编程的T细胞的转移增强了对细胞内病原体的免疫保护,将T细胞群转移到短寿命的效应细胞.

结论:

微RNA的纳米线介导输送提供了一个新的策略,可以在没有预先激活的情况下对原始T细胞进行编程,从而提高它们的治疗潜力.
这种方法调节T细胞分化并增强体内免疫反应,为采用T细胞治疗开发提供了一个有前途的途径.

更多相关视频

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Published on: April 13, 2017

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

Published on: December 26, 2015

相关概念视频

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...