糖尿病巨外体miR-381-3p通过NR5A2抑制表皮细胞自
Xin Huang1, Linhesheng Wei1, Mengdi Li2
1Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.
International dental journal
|April 29, 2024
概括
糖尿病小鼠巨细胞的外体通过miR-381-3p破坏牙细胞的自,抑制NR5A2并增加炎症. 这揭示了糖尿病牙周炎的一个关键机制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 糖尿病与牙周炎症的增加有关.
- 自在维持细胞平衡和免疫反应方面发挥着至关重要的作用.
- 牙上皮细胞 (Gengival Epithelial Cells,GECs) 中自的破坏可能导致糖尿病并发症.
研究的目的:
- 阐明糖尿病患者的GEC中自性被破坏的机制.
- 研究骨髓衍生巨细胞 (BMDMs) 衍生出来的外体在这个过程中的作用.
主要方法:
- 从糖尿病 (db/db) 和对照 (C57/bl) 小鼠的BMDM中分离出外部体 (Exo).
- 使用qRT-PCR和西式斑点检测,与外体组共同培养GEC,并分析自标记物 (例如ATG7).
- 生物信息分析 (AnimalTFDB,TargetScan,miRDB,miRWalk) 用于识别转录因子和miRNAs.
- 使用siRNA,miRNA模仿剂/抑制剂和双露西法酶试验的功能验证.
主要成果:
- 糖尿病BMDMs (ExoDM) 的外体破坏了自并促进了GECs的炎症.
- 确定NR5A2是调节ATG7表达的关键转录因子;其抑制加剧了炎症.
- 确定miR-381-3p是主要的miRNA向NR5A2;其抑制恢复了自和减少了炎症.
结论:
- 来自糖尿病BMDM的外体携带miR-381-3p,它抑制了GEC中的NR5A2.
- 这种抑制会破坏自,导致糖尿病患者牙周炎症增加.
- 这一miR-381-3p/NR5A2/ATG7轴代表了糖尿病牙周炎发病的关键途径.
相关概念视频
Autophagy
4.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K
Delivery Pathways to the Lysosome
6.4K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.4K
Phagocytosis of Apoptotic Cells
3.8K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.8K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K


