一种C型莱克 (CTL2) 调解了Tribolium castaneum中对细菌感染的幽默和细胞免疫力
Suisui Wang1, Shiyuan Miao2, Yujie Lu2
1Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
红色面粉甲虫中的C型莱克2 (CTL2) 增强了对细菌感染的细胞和幽默免疫力. 击败CTL2会增加死亡率和细菌负载,揭示了它在天生的免疫力中的关键作用.
科学领域:
- 免疫学 免疫学 免疫学
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
背景情况:
- C型讲蛋白 (CTLs) 对于无脊椎动物的免疫反应至关重要,在Tribolium castaneum的幽默免疫中具有已知的作用.
- 在 T. castaneum 的细胞免疫中,CTL 的特定功能是一个重要的存储产品害虫,仍然在很大程度上没有表征.
研究的目的:
- 为了识别和表征一种新的C型瘦素基因,指定CTL2 (TcCTL2),在Tribolium castaneum.
- 阐明TCCTL2在针对细菌病原体的幽默和细胞免疫反应中的作用.
主要方法:
- 对TCCTL2基因的识别和序列分析.
- 通过定量实时PCR和现场杂交进行基因表达分析.
- 功能性研究涉及基因淘汰 (RNAi),重组蛋白净化和体外免疫试验 (封装,黑色化,细胞化,凝结).
- 在TcCTL2沉默和细菌挑战后,对免疫路径基因表达的分析.
主要成果:
- TcCTL2编码了一种具有碳水化合物识别域的分泌蛋白质,主要在晚期幼虫阶段在血细胞中表达.
- 在细菌感染 (大肠杆菌,金黄色葡萄球菌) 时,TCCTL2表达显著上调.
- 抑制TcCTL2导致幼虫死亡率增加,细菌殖民增强,以及血液细胞介导的细胞免疫反应受损 (封装,黑色化,细胞化).
- 重组TcCTL2以依赖的方式促进细菌凝结,并在体外增强细胞形成和封装.
- 沉默TCCTL2抑制了关键免疫路径基因 (Toll,IMD),抗菌和氧化酶活性.
结论:
- 在Tribolium castaneum中,TCCTL2在调解幽默性和细胞性免疫力方面发挥着关键的双重作用,对于细菌清除至关重要.
- 这些发现加深了对昆虫天生的免疫力中CTL功能的理解,并提供了利用RNAi和感染模型优化害虫控制策略的见解.
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