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Tengfei Xiong¹, Mingyue Yu¹, Jiang Zhu¹

¹State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Pesticide biochemistry and physiology

|April 30, 2024

概括

一个新发现的P450基因,HassCYP6B6,在东方烟草虫中代谢了传统的杀虫剂,如有机酸盐和铁酸盐. 这种害虫管理洞察力有助于了解抗虫剂耐药性和应用策略.

科学领域:

昆虫学昆虫学是一门学科.
生物化学生物化学
虫害管理虫害管理虫害管理

背景情况:

东方烟草虫 (Helicoverpa assulta) 是一个重要的农业害虫,对和烟草等作物造成损害.
害虫对杀虫剂的耐药性需要了解杀虫剂转化的生化机制.

研究的目的:

为了确定参与Helicoverpa assulta.在杀虫剂代谢中的关键酶.
描述特定的P450基因HassCYP6B6对各种杀虫剂的活性.

主要方法:

在Helicoverpa assulta.中识别和表达CYP6B亚系P450基因 (HassCYP6B6) 的表达分析.
使用重组HassCYP6B6酶对12种不同的杀虫剂进行体外代谢试验.
使用质谱学阐明代谢物结构.

主要成果:

哈斯CYP6B6对有机酸盐 (迪亚津,马拉,福西姆),酸盐 (普罗普克苏尔) 和酸盐 (埃斯芬瓦莱拉特) 具有显著的代谢活性.
对于新烟类药物,二化物,宏环乳或甲胺没有观察到任何显著的代谢.
代谢途径包括脱,化和氧化脱硫.

结论:

在Helicoverpa assulta中,HassCYP6B6在几种常规杀虫剂的排毒中发挥着至关重要的作用.
了解HassCYP6B6的基质特异性,可以深入了解昆虫剂耐药性机制.
这些发现对优化杀虫剂使用和开发耐药性管理策略有影响.

关键词:

细胞染色体P450单氧化原酶.脱水化的过程脱硫处理脱硫处理氧化液的氧化方式东方烟草虫是一种东方烟草虫.再组合功能表达的功能表达

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