阿基林2通过与V. splendidus挑战的Apostichopus japonicus中的14-3-3ζ相互作用来增强抗菌能力
Wenjun Tao1, Xin Li1, Xianmu Fu1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China.
Fish & shellfish immunology
|April 30, 2024
概括
阿基林2在海黄的免疫力中起着至关重要的作用,对抗Vibrio splendidus感染. 这种蛋白与Aj14-3-3ζ相互作用,增强抗菌防御和调节炎症反应.
科学领域:
- 海洋生物学 海洋生物学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 已知Akirin2可以调节脊椎动物的免疫反应.
- 在无脊椎动物中Akirin2的功能,特别是天生的免疫力,基本上是未知的.
- 海是重要的海洋无脊椎动物,具有重要的生态作用.
研究的目的:
- 克隆和描述来自A. japonicus (AjAkirin2) 的阿基林2基因.
- 为了阐明AjAkirin2在对Vibrio splendidus感染的反应中的免疫机制.
- 研究AjAkirin2与其他免疫分子的相互作用及其在NF-κB途径中的作用.
主要方法:
- 克隆AJAkirin2cDNA并进行序列分析.
- 对AjAkirin2mRNA和蛋白质的空间表达分析.
- 用RNA干扰进行基因淘汰实验.
- GST-pull down和共同免疫沉降试验用于研究蛋白质相互作用.
- 与NF-κB途径和炎症性细胞因子相关的基因表达的分析.
主要成果:
- AjAkirin2在海黄组织中构成性表达,在细菌挑战时在胆红细胞中表达高.
- 击败AJAkirin2显著降低了感染Vibrio splendidus的海的存活率.
- AjAkirin2直接与Aj14-3-3ζ相互作用,并积极调节其表达.
- 击败AjAkirin2或Aj14-3-3ζ增加了细胞内细菌负载,并抑制了关键的免疫和炎症基因表达.
结论:
- AjAkirin2对于海对Vibrio splendidus的天生的免疫反应至关重要.
- 阿杰克林2的抗菌功能是通过其与AJ14-3-3ζ.的相互作用来调节的.
- AjAkirin2在调节NF-κB信号通路和炎症性细胞因子产生方面发挥作用.
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