测量方法的比较,用于量化性激素度在月经周期的 rhesus macaques†
Kristopher M Fecteau1, Andrea J Winchell1, Steven W Blue1
1Endocrine Technologies Core, Oregon National Primate Research Center, Beaverton, OR, USA.
Biology of reproduction
|April 30, 2024
概括
自动免疫测试 (AIA) 和液态染色体-并联质谱 (LC-MS/MS) 显示出在 rhesus macaque 血清中分析17β-雌激素和孕激素的良好一致性. 然而,对于精确的类固醇激素量化,当AIA可能高估或低估水平时,LC-MS/MS是首选的.
科学领域:
- 类固醇激素量化方法的比较分析.
- 灵长类生殖内分泌学.
- 分析化学和测试验证.
背景情况:
- 自动免疫试验 (AIAs) 广泛用于类固醇激素分析,因为其产量高,成本低.
- 液体染色学-并联质谱学 (LC-MS/MS) 为类固醇量化提供了卓越的特异性,选择性和复杂化能力.
- 精确监测类固醇激素的特征对于理解生殖周期至关重要.
研究的目的:
- 为了比较AIA和LC-MS/MS的性能,在 rhesus的血清中量化17β-雌激醇 (E2) 和孕 (P4).
- 评估协议并确定两种分析方法之间的潜在偏差.
- 评估每个方法在生殖研究中的不同应用的适用性.
主要方法:
- 通过多个月经周期分析了12只 rhesus macaque 的血清样本.
- 使用罗氏科巴斯e411分析仪 (AIA) 和Shimadzu Nexera LC-MS/MS仪器测量了17β-雌激素和孕激素度.
- 统计分析包括通过巴布洛克回归和布兰德-阿尔特曼情节来评估协议和偏见.
主要成果:
- 在AIA和LC-MS/MS之间观察到E2和P4的优异一致性,而Bland-Altman图表没有发现显著偏差.
- 与LC-MS/MS相比,AIA倾向于高估E2在度>140pg/ml时,低估P4在度>4ng/ml时.
- 对于丸激素,发现了显著的差异,AIA始终低估了相对于LC-MS/MS的水平.
结论:
- AIA适用于常规监测灵长类动物的月经周期或当需要快速单个数据点时.
- LC-MS/MS提供了更准确的类固醇激素量化,特别是在更高度的E2和P4,并且在精度至关重要的情况下是可取的.
- 选择方法应考虑具体的应用,所需的准确性和正在分析的类固醇的度范围.
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