Circ_0003855涉及食道癌的进展通过miR-622/FLOT1进行

相关实验视频

Updated: Jun 27, 2025

Development of Compendium for Esophageal Squamous Cell Carcinoma

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

Jingjing Tian¹, Xibao Hu², Xinrong Zhang¹

¹Department of Gastroenterology, Tianjin Nankai Hospital, Tianjin, 300100, China.

Oncology research

|April 30, 2024

概括

循环RNA Circ_0003855通过降低miR-622的调节和提高FLOT1.1的调节来促进食道癌 (EC) 细胞活性. 抑制Circ_0003855降低了EC细胞的活性,提供了一个潜在的治疗点.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
基因规则基因规则

背景情况:

食道癌 (EC) 是一个重要的全球健康问题.
循环RNAs (circRNAs) 在EC病变发生中的作用越来越被认可.
Circ_0003855已与各种癌症有关,但其在EC中的特定功能需要阐明.

研究的目的:

调查Circ_0003855在食道癌 (EC) 中的作用.
探索涉及miR-622和FLOT1在EC进展中的分子机制.
评估EC中针对Circ_0003855的治疗潜力.

主要方法:

在EC细胞 (Eca109) 和正常细胞 (HEEC) 中分析Circ_0003855,miR-622和FLOT1的表达.
使用小干扰RNA (si-Circ_0003855) 的功能测试来评估细胞活动的变化.
与miR-622抑制剂的同传染实验以阐明调节途径.

主要成果:

在EC细胞中,Circ_0003855和FLOT1的调节上升,而miR-622的调节下降.
si-Circ_0003855传染减少了EC细胞活性,增加了miR-622,并减少了FLOT1表达.
与miR-622抑制剂的同时感染消除了si-Circ_0003855对FLOT1表达的影响.

结论:

Circ_0003855通过miR-622/FLOT1轴促进EC细胞活动.
向Circ_0003855可能代表了食道癌的新疗法策略.
需要进一步的研究来验证这些发现在临床环境中.

关键词:

细胞的增殖细胞的增殖.圆周_0003855 在线观看食道癌是食道癌的一个类型.在FLOT1上.据报道,米R-62222是该公司的产品.

更多相关视频

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids

Published on: December 23, 2022

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...