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对Ophiocordyceps unilateralis sensu lato进行基因组比较分析

Yingling Lu^1,2, Dexiang Tang^1,2, Zuoheng Liu^1,2

¹Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, China.

Frontiers in microbiology

|April 30, 2024

概括

这项研究描述了一种新的病原菌,Ophiocordyceps fusiformispora,并分析了12种Ophiocordyceps unilateralis物种的基因组. 它揭示了二次代谢物基因集群中的显著差异,有助于未来对真菌化合物的研究.

科学领域:

菌类学菌类学是指菌类学.
基因组学就是基因组学.
生物化学生物化学

背景情况:

甲状腺 (Ophiocordyceps unilateralis sensu lato) 是一个重要的病原体.
以前的研究主要集中在形态学和有限的遗传标记物上.

研究的目的:

为了描述一个新物种,Ophiocordyceps fusiformispora.
为了进行12种Ophiocordyceps unilateralis物种的比较基因组学.
为了确定二次代谢物生物合成基因集群和潜在的化合物.

主要方法:

使用五个基因 (SSU,LSU,TEF1α,RPB1,RPB2) 的形态学和遗传学分析.
七种Ophiocordyceps物种的全基因组测序和注释.
对二次代谢物基因集群进行比较基因组分析,包括AntiSMASH和局部BLAST.
使用最大概率 (ML) 和贝叶斯推理 (BI) 树进行的家族遗传学分析.

主要成果:

一个新的物种Ophiocordyceps fusiformispora被发现.
全基因组特征 (GC含量,基因组大小) 在相关物种中相似.
在物种之间观察到二次代谢物生物合成基因集群 (SM BGCs) 的显著变化,包括NRPS,PKS和混合PKS-NRPS域.
对于五种化合物 (NG-391,lucilactaene,higginsianin B,pyripyropene A,pyranonigrin E) 的假定BGCs被确定.
鉴定出NG-391和lucilactaene是富沙林C的7 - 脱甲基类似物.
在12个基因组中发现了共同的基因域 (例如KS-AT-DH-MT-ER-KR-ACP).
基于特定基因域的家族遗传树与多基因家族遗传相一致.

结论:

对比基因组学为Ophiocordyceps unilateralis中二次新陈代谢的演变提供了宝贵的见解.
该研究确定了关键的二次代谢物及其遗传基础.
建立了一种获得活体培养和无性形式的方法,促进未来对Ophiocordyceps unilateralis及其代谢物的研究.

关键词:

生物合成基因集群生物合成基因集群基因开采是指基因开采的过程.一个新的分类.二次代谢产物二次代谢产物整个基因组序列的测序.

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