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在冷解过程后,与胞相关的miRNAs与阿斯动物的霍尔斯坦公牛的功能和流量细胞计参数相关

Morteza Taravat¹, Amirmahdi Roshanzamir², Maryam Rahbar¹

¹Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.

Biopreservation and biobanking

|April 30, 2024

概括

微RNAs调节精子的功能. 这项研究确定了与不孕症的原因 - - 精子精症 (AS) 相关的特定微RNA,以及它们与精子运动性和亡的关联.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

精子细胞的细胞过程,如亡和运动,由微RNAs (miRNAs) 调节.
精子生育不良症 (AS),以精子运动性降低为特征,是男性不孕症的重要原因之一.
特定的miRNA和分子通路都与AS病变发生有关.

研究的目的:

为了研究与亡相关的miRNAs在霍尔斯坦公牛的精 (AS) 中的作用.
为了确定与精子功能和AS中的亡相关的特定miRNAs与normozoospermia (NS) 相比.

主要方法:

从霍尔斯坦牛群中采集了患有常态精 (NS) 和精精 (AS) 的精液样本,并进行了冷保存.
评估了精子运动参数,等离子体膜完整性,体完整性,DNA碎片化和亡状态.
使用生物信息学方法分析了与亡相关的特定miRNAs (miR-2114,miR-296-3p,miR-455-3p和miR345-3p) 的表达水平.

主要成果:

与AS组相比,NS组的功能和流量细胞计参数在NS组显著改善.
在NS组中,miR-455-3pp和miR-2412的上调,而miR-345-3p相对于AS组的下调.
在AS组中,miR-296-39,miR-2412,miR-345-3p水平与膜完整性,DNA碎片化和亡状态有很强的相关性.

结论:

通过生物信息学分析识别的精选miRNA与AS和NS样本中的功能和流动细胞计量指标有很大联系.
这些miRNAs可能在调节亡和精中的运动性方面发挥关键作用.
这些发现有助于理解AS背后的分子机制,并为生育干预提供潜在的目标.

关键词:

灭症 (apoptosis) 是一种死亡的过程.阿斯动物精子缺血症在公牛的牛,公牛的牛.这是一个小RNARNA.常态动物精子症 (normozoospermia) 是一种常态动物精子症.

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