使用纳米孔对未充电的的电学感知,并使用纳米孔对它们的化状态进行区分
Wei Si1, Jiayi Chen1, Zhen Zhang1
1Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, 211100, China.
概括
这项研究表明使用带电石墨烯纳米孔捕获和感知未带电. 这一进步使得低成本,高通量测序蛋白质残留物及其翻译后修改成为可能.
科学领域:
- 生物物理学的生物物理.
- 纳米技术纳米技术
- 蛋白质组学是指蛋白质组学.
背景情况:
- 准确的蛋白质表征对于理解生物功能和基因组分析至关重要.
- 虽然纳米孔技术在核酸测序方面表现出色,但快速蛋白质测序,特别是未加电的蛋白质,仍然具有挑战性.
- 石墨烯纳米孔为检测酸化等蛋白质修饰提供了高精度,灵敏度和成本效益.
研究的目的:
- 开发一种使用带电石墨烯纳米孔捕获和感知未带电的方法.
- 使用基于纳米孔的技术,区分未充电的和它们的化状态.
- 评估蛋白质残留物和翻译后修改的低成本,高通量测序的潜力.
主要方法:
- 用全原子分子动力学模拟来模拟带电石墨烯纳米孔内的类相互作用.
- 电力被利用来捕获和感知未充电的.
- 原子力显微镜 (AFM) 操纵被模仿在纳米孔通道期间分析单个分子.
主要成果:
- 充电的石墨烯纳米孔通过电流成功捕获和感知未充电的.
- 离子电流和拉力信号都在纳米孔转移过程中将未充电的与其酸化形式区分开来.
- 该研究证实了检测和区分单个氨基酸及其酸化状态的能力.
结论:
- 带电的石墨烯纳米孔提供了一种可行的方法,用于电学捕获和感知未带电的.
- 纳米孔分析,结合力测量,可以区分酸化状态.
- 这种方法对未来的低成本,高通量蛋白质测序和翻译后修改分析具有重大前景.
相关概念视频
01:15Potentiometry: Membrane Electrodes
567
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
567
01:20Capillary Electrophoresis: Instrumentation
228
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
228
01:30Capillary Electrophoresis: Applications
394
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
394