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在临床制造的BM-MSC产品中使用SDS-PAGE进行残留蛋白质分析

Pelin Kilic1,2, Sema Karabudak3,4, Begum Cosar2,5

  • 1Department of Stem Cells and Regenerative Medicine, Stem Cell Institute, Ankara University, Ankara, Turkey.

Electrophoresis
|April 30, 2024
PubMed
概括

这项研究验证了二甲基硫酸盐-多烯胺凝电泳 (SDS-PAGE) 作为分析细胞治疗中的残留物质的成本有效方法. 这些发现指导制造工厂采用临床产品可访问的质量控制措施.

关键词:
先进疗法的药物,先进疗法的药物.骨髓衍生中细胞/干细胞 (BM-MSCs)介酶体干细胞/干细胞 (MSCs)剩余蛋白质的分析.二甲基硫酸盐聚烯胺凝电泳 (SDS-PAGE) 的方法

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相关概念视频

SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
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